Quantitative analysis of Epstein-Barr virus (EBV)-related gene expression in patients with chronic active EBV infection

1 Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan 2 Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan 3 Division of Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan 4 Department of Virology, Division of Medica...

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Bibliographic Details
Published in:Journal of general virology 2010-01, Vol.91 (1), p.42-50
Main Authors: Iwata, Seiko, Wada, Kaoru, Tobita, Satomi, Gotoh, Kensei, Ito, Yoshinori, Demachi-Okamura, Ayako, Shimizu, Norio, Nishiyama, Yukihiro, Kimura, Hiroshi
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biological and medical sciences
Child
Child, Preschool
Epstein-Barr Virus Infections - virology
Female
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Gene Expression Regulation, Viral
Genetics
Herpesvirus 4, Human - genetics
Herpesvirus 4, Human - physiology
Humans
Leukocytes, Mononuclear - virology
Male
Microbiology
Miscellaneous
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
RNA, Viral - biosynthesis
RNA, Viral - genetics
Transcription, Genetic
Virology
Young Adult
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Summary:1 Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan 2 Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan 3 Division of Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan 4 Department of Virology, Division of Medical Science, Medical Research Institute, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan Correspondence Hiroshi Kimura hkimura{at}med.nagoya-u.ac.jp Chronic active Epstein–Barr virus (CAEBV) infection is a systemic Epstein–Barr virus (EBV)-positive lymphoproliferative disorder characterized by persistent or recurrent infectious mononucleosis-like symptoms in patients with no known immunodeficiency. The detailed pathogenesis of the disease is unknown and no standard treatment regimen has been developed. EBV gene expression was analysed in peripheral blood samples collected from 24 patients with CAEBV infection. The expression levels of six latent and two lytic EBV genes were quantified by real-time RT-PCR. EBV-encoded small RNA 1 and Bam HI-A rightward transcripts were abundantly detected in all patients, and latent membrane protein (LMP) 2 was observed in most patients. EBV nuclear antigen (EBNA) 1 and LMP1 were detected less frequently and were expressed at lower levels. EBNA2 and the two lytic genes were not detected in any of the patients. The pattern of latent gene expression was determined to be latency type II. EBNA1 was detected more frequently and at higher levels in the clinically active patients. Quantifying EBV gene expression is useful in clarifying the pathogenesis of CAEBV infection and may provide information regarding a patient's disease prognosis, as well as possible therapeutic interventions. A supplementary table of primer sequences is available with the online version of this paper.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.013482-0