Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat

The aspartic protease (FeAP9) gene from buckwheat resembles the exon–intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5′-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs:...

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Bibliographic Details
Published in:Journal of plant physiology 2010, Vol.167 (1), p.61-68
Main Authors: TIMOTIJEVIC, Gordana S, MILISAVLJEVIC, Mira D. J, RADOVIC, Svetlana R, KONSTANTINOVIC, Miroslav M, MAKSIMOVIC, Vesna R
Format: Article
Language:English
Subjects:
Abiotic stress
Aspartic Acid Endopeptidases - genetics
Aspartic Acid Endopeptidases - metabolism
Aspartic proteinase
aspartic proteinases
Base Sequence
Biological and medical sciences
Buckwheat
drought
Fagopyrum - enzymology
Fagopyrum - genetics
Fagopyrum - physiology
Fagopyrum esculentum
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Profiling
gene expression regulation
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genes, Plant
Leader intron
light intensity
Molecular Sequence Data
plant damage
Plant physiology and development
plant response
plant stress
salicylic acid
Stress, Physiological
tissue distribution
ultraviolet radiation
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Summary:The aspartic protease (FeAP9) gene from buckwheat resembles the exon–intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5′-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, seedlings, flowers, leaves, roots and stems. Using Real-time PCR, we found that FeAP9 expression is upregulated in buckwheat leaves under the influence of different abiotic stresses, including dark, drought and UV-B light, as well as wounding and salicylic acid.
ISSN:0176-1617
1618-1328
DOI:10.1016/j.jplph.2009.06.017