Determination of ethylenediaminetetra-acetic acid in aqueous rinses of detergent-washed rubber stoppers of pharmaceutical vials using solid-phase extraction and capillary gas chromatography

A fused silica capillary gas chromatographic method is presented for the determination of traces of ethylenediaminetetra-acetic acid (EDTA) in aqueous rinses of rubber stoppers of pharmaceutical vials after treatment with detergents containing EDTA. Isolation and enrichment of EDTA from the aqueous...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1987, Vol.5 (7), p.687-694
Main Authors: Ribick, Michael A., Jemal, Mohammed, Cohen, Allen I.
Format: Article
Language:English
Subjects:
Analysis
anion exchange
Biological and medical sciences
capillary GC
Ethylenediaminetetra-acetic acid
General pharmacology
Medical sciences
Pharmacology. Drug treatments
rubber stoppers of vials
solid-phase extraction
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Summary:A fused silica capillary gas chromatographic method is presented for the determination of traces of ethylenediaminetetra-acetic acid (EDTA) in aqueous rinses of rubber stoppers of pharmaceutical vials after treatment with detergents containing EDTA. Isolation and enrichment of EDTA from the aqueous medium is achieved using a commercially available strong anion-exchange solid-phase extraction cartridge, transformed to the formate form. A 2.0-ml volume of methanolic HCl is used for both elution of EDTA from the extraction column and formation of the tetramethyl ester derivative. With the incorporation of a methanolic wash to eliminate interfering components prior to elution with methanolic HCl, a limit of detection of 25 ng EDTA per ml water with a non-selective flame ionization detector is possible.
ISSN:0731-7085
1873-264X
DOI:10.1016/0731-7085(87)80081-X