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Protective effect of erythropoietin on torsion/detorsion injury in rat model
Abstract Purpose The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats. Methods Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D1 ), 3 hours ischemia and 1 hour reperfusion; group...
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| Published in: | Journal of pediatric surgery 2009-10, Vol.44 (10), p.1988-1994 |
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| container_end_page | 1994 |
| container_issue | 10 |
| container_start_page | 1988 |
| container_title | Journal of pediatric surgery |
| container_volume | 44 |
| creator | Bakan, Vedat Çıralık, Harun Tolun, Fatma İnanaç Atlı, Yalçın Mil, Ayhan Öztürk, Şenol |
| description | Abstract Purpose The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats. Methods Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D1 ), 3 hours ischemia and 1 hour reperfusion; group III (torsion/detorsion 2, T/D2 ), 3 hours ischemia and 48 hours reperfusion; group IV (erythropoietin 1, EPO1 ), 3 hours ischemia, 1 hour reperfusion, and a single dose of EPO; and group V (erythropoietin 2, EPO2 ), 3 hours ischemia, 48 hours reperfusion, and 2 doses of EPO. Malondialdehyde (MDA) and nitric oxide (NO) levels and activities of superoxide dismutase and catalase were measured. Tissue damage to ovarian tissue was scored by histologic examination. Data were compared among groups with parametric tests. Results The MDA levels in the S and EPO groups were significantly lower than the T/D groups ( P .05). Conclusion Erythropoietin attenuates ischemia-reperfusion injury when given during the acute phase of ovarian torsion-detorsion in a rat model. |
| doi_str_mv | 10.1016/j.jpedsurg.2009.02.071 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_734101685</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022346809001572</els_id><sourcerecordid>734101685</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-7350e799e0045c10a79eb70bf096fd57eecc42fee1b758772c76ba375e5c89933</originalsourceid><addsrcrecordid>eNqFkU9LxDAQxYMouq5-BenNU7uTZNNsLqKI_2BBQT2HNp1qardZk1bYb2_KrghePM0wvDfD-w0hZxQyCjSfNVmzxioM_i1jACoDloGke2RCBaepAC73yQSAsZTP88UROQ6hAYhjoIfkiKqF4DKHCVk-edej6e0XJljXsUtcnaDf9O_erZ3F3naJ65Le-WBdN6tw1yW2awa_iSXxRZ-sXIXtCTmoizbg6a5Oyevtzcv1fbp8vHu4vlqmZs5Yn0ouAKVSCDAXhkIhFZYSyhpUXldCIpoorBFpKcVCSmZkXhZcChRmoRTnU3K-3bv27nPA0OuVDQbbtujQDUFLPh8hxYxTkm-VxrsQPNZ67e2q8BtNQY8a3egfkHoEqYHpCDIaz3YnhnKF1a9tRy4KLrcCjEG_LHodjMXOYGV9pKgrZ_-_cfFnhWltZ03RfuAGQ-MG30WMmuoQDfp5fOf4TVAAVEjGvwGtJZ2I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>734101685</pqid></control><display><type>article</type><title>Protective effect of erythropoietin on torsion/detorsion injury in rat model</title><source>ScienceDirect Freedom Collection</source><creator>Bakan, Vedat ; Çıralık, Harun ; Tolun, Fatma İnanaç ; Atlı, Yalçın ; Mil, Ayhan ; Öztürk, Şenol</creator><creatorcontrib>Bakan, Vedat ; Çıralık, Harun ; Tolun, Fatma İnanaç ; Atlı, Yalçın ; Mil, Ayhan ; Öztürk, Şenol</creatorcontrib><description>Abstract Purpose The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats. Methods Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D1 ), 3 hours ischemia and 1 hour reperfusion; group III (torsion/detorsion 2, T/D2 ), 3 hours ischemia and 48 hours reperfusion; group IV (erythropoietin 1, EPO1 ), 3 hours ischemia, 1 hour reperfusion, and a single dose of EPO; and group V (erythropoietin 2, EPO2 ), 3 hours ischemia, 48 hours reperfusion, and 2 doses of EPO. Malondialdehyde (MDA) and nitric oxide (NO) levels and activities of superoxide dismutase and catalase were measured. Tissue damage to ovarian tissue was scored by histologic examination. Data were compared among groups with parametric tests. Results The MDA levels in the S and EPO groups were significantly lower than the T/D groups ( P < .001). Catalase and superoxide dismutase activities, and NO levels in the S and EPO groups were significantly higher than in the T/D groups ( P < .05). Ovarian tissue damage in the S and EPO groups was significantly less than in the T/D groups ( P < .05). Levels of all biochemical markers and ovarian tissue damage scores were similar among the S, EPO1 , and EPO2 groups ( P > .05). Conclusion Erythropoietin attenuates ischemia-reperfusion injury when given during the acute phase of ovarian torsion-detorsion in a rat model.</description><identifier>ISSN: 0022-3468</identifier><identifier>EISSN: 1531-5037</identifier><identifier>DOI: 10.1016/j.jpedsurg.2009.02.071</identifier><identifier>PMID: 19853760</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antioxidants - therapeutic use ; Catalase - metabolism ; Disease Models, Animal ; Erythropoietin ; Erythropoietin - therapeutic use ; Female ; Ischemia-reperfusion ; Malondialdehyde - metabolism ; Nitric Oxide - metabolism ; Ovarian Diseases - metabolism ; Ovarian Diseases - pathology ; Ovarian Diseases - prevention & control ; Ovarian torsion-detorsion ; Ovary - metabolism ; Ovary - pathology ; Pediatrics ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins ; Reperfusion Injury - metabolism ; Reperfusion Injury - pathology ; Reperfusion Injury - prevention & control ; Superoxide Dismutase - metabolism ; Surgery ; Torsion Abnormality - drug therapy ; Torsion Abnormality - metabolism ; Torsion Abnormality - pathology ; Torsion Abnormality - prevention & control</subject><ispartof>Journal of pediatric surgery, 2009-10, Vol.44 (10), p.1988-1994</ispartof><rights>Elsevier Inc.</rights><rights>2009 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-7350e799e0045c10a79eb70bf096fd57eecc42fee1b758772c76ba375e5c89933</citedby><cites>FETCH-LOGICAL-c422t-7350e799e0045c10a79eb70bf096fd57eecc42fee1b758772c76ba375e5c89933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19853760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bakan, Vedat</creatorcontrib><creatorcontrib>Çıralık, Harun</creatorcontrib><creatorcontrib>Tolun, Fatma İnanaç</creatorcontrib><creatorcontrib>Atlı, Yalçın</creatorcontrib><creatorcontrib>Mil, Ayhan</creatorcontrib><creatorcontrib>Öztürk, Şenol</creatorcontrib><title>Protective effect of erythropoietin on torsion/detorsion injury in rat model</title><title>Journal of pediatric surgery</title><addtitle>J Pediatr Surg</addtitle><description>Abstract Purpose The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats. Methods Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D1 ), 3 hours ischemia and 1 hour reperfusion; group III (torsion/detorsion 2, T/D2 ), 3 hours ischemia and 48 hours reperfusion; group IV (erythropoietin 1, EPO1 ), 3 hours ischemia, 1 hour reperfusion, and a single dose of EPO; and group V (erythropoietin 2, EPO2 ), 3 hours ischemia, 48 hours reperfusion, and 2 doses of EPO. Malondialdehyde (MDA) and nitric oxide (NO) levels and activities of superoxide dismutase and catalase were measured. Tissue damage to ovarian tissue was scored by histologic examination. Data were compared among groups with parametric tests. Results The MDA levels in the S and EPO groups were significantly lower than the T/D groups ( P < .001). Catalase and superoxide dismutase activities, and NO levels in the S and EPO groups were significantly higher than in the T/D groups ( P < .05). Ovarian tissue damage in the S and EPO groups was significantly less than in the T/D groups ( P < .05). Levels of all biochemical markers and ovarian tissue damage scores were similar among the S, EPO1 , and EPO2 groups ( P > .05). Conclusion Erythropoietin attenuates ischemia-reperfusion injury when given during the acute phase of ovarian torsion-detorsion in a rat model.</description><subject>Animals</subject><subject>Antioxidants - therapeutic use</subject><subject>Catalase - metabolism</subject><subject>Disease Models, Animal</subject><subject>Erythropoietin</subject><subject>Erythropoietin - therapeutic use</subject><subject>Female</subject><subject>Ischemia-reperfusion</subject><subject>Malondialdehyde - metabolism</subject><subject>Nitric Oxide - metabolism</subject><subject>Ovarian Diseases - metabolism</subject><subject>Ovarian Diseases - pathology</subject><subject>Ovarian Diseases - prevention & control</subject><subject>Ovarian torsion-detorsion</subject><subject>Ovary - metabolism</subject><subject>Ovary - pathology</subject><subject>Pediatrics</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Recombinant Proteins</subject><subject>Reperfusion Injury - metabolism</subject><subject>Reperfusion Injury - pathology</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Surgery</subject><subject>Torsion Abnormality - drug therapy</subject><subject>Torsion Abnormality - metabolism</subject><subject>Torsion Abnormality - pathology</subject><subject>Torsion Abnormality - prevention & control</subject><issn>0022-3468</issn><issn>1531-5037</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkU9LxDAQxYMouq5-BenNU7uTZNNsLqKI_2BBQT2HNp1qardZk1bYb2_KrghePM0wvDfD-w0hZxQyCjSfNVmzxioM_i1jACoDloGke2RCBaepAC73yQSAsZTP88UROQ6hAYhjoIfkiKqF4DKHCVk-edej6e0XJljXsUtcnaDf9O_erZ3F3naJ65Le-WBdN6tw1yW2awa_iSXxRZ-sXIXtCTmoizbg6a5Oyevtzcv1fbp8vHu4vlqmZs5Yn0ouAKVSCDAXhkIhFZYSyhpUXldCIpoorBFpKcVCSmZkXhZcChRmoRTnU3K-3bv27nPA0OuVDQbbtujQDUFLPh8hxYxTkm-VxrsQPNZ67e2q8BtNQY8a3egfkHoEqYHpCDIaz3YnhnKF1a9tRy4KLrcCjEG_LHodjMXOYGV9pKgrZ_-_cfFnhWltZ03RfuAGQ-MG30WMmuoQDfp5fOf4TVAAVEjGvwGtJZ2I</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Bakan, Vedat</creator><creator>Çıralık, Harun</creator><creator>Tolun, Fatma İnanaç</creator><creator>Atlı, Yalçın</creator><creator>Mil, Ayhan</creator><creator>Öztürk, Şenol</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>Protective effect of erythropoietin on torsion/detorsion injury in rat model</title><author>Bakan, Vedat ; Çıralık, Harun ; Tolun, Fatma İnanaç ; Atlı, Yalçın ; Mil, Ayhan ; Öztürk, Şenol</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-7350e799e0045c10a79eb70bf096fd57eecc42fee1b758772c76ba375e5c89933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Antioxidants - therapeutic use</topic><topic>Catalase - metabolism</topic><topic>Disease Models, Animal</topic><topic>Erythropoietin</topic><topic>Erythropoietin - therapeutic use</topic><topic>Female</topic><topic>Ischemia-reperfusion</topic><topic>Malondialdehyde - metabolism</topic><topic>Nitric Oxide - metabolism</topic><topic>Ovarian Diseases - metabolism</topic><topic>Ovarian Diseases - pathology</topic><topic>Ovarian Diseases - prevention & control</topic><topic>Ovarian torsion-detorsion</topic><topic>Ovary - metabolism</topic><topic>Ovary - pathology</topic><topic>Pediatrics</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Recombinant Proteins</topic><topic>Reperfusion Injury - metabolism</topic><topic>Reperfusion Injury - pathology</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Surgery</topic><topic>Torsion Abnormality - drug therapy</topic><topic>Torsion Abnormality - metabolism</topic><topic>Torsion Abnormality - pathology</topic><topic>Torsion Abnormality - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bakan, Vedat</creatorcontrib><creatorcontrib>Çıralık, Harun</creatorcontrib><creatorcontrib>Tolun, Fatma İnanaç</creatorcontrib><creatorcontrib>Atlı, Yalçın</creatorcontrib><creatorcontrib>Mil, Ayhan</creatorcontrib><creatorcontrib>Öztürk, Şenol</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pediatric surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bakan, Vedat</au><au>Çıralık, Harun</au><au>Tolun, Fatma İnanaç</au><au>Atlı, Yalçın</au><au>Mil, Ayhan</au><au>Öztürk, Şenol</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of erythropoietin on torsion/detorsion injury in rat model</atitle><jtitle>Journal of pediatric surgery</jtitle><addtitle>J Pediatr Surg</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>44</volume><issue>10</issue><spage>1988</spage><epage>1994</epage><pages>1988-1994</pages><issn>0022-3468</issn><eissn>1531-5037</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Abstract Purpose The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats. Methods Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D1 ), 3 hours ischemia and 1 hour reperfusion; group III (torsion/detorsion 2, T/D2 ), 3 hours ischemia and 48 hours reperfusion; group IV (erythropoietin 1, EPO1 ), 3 hours ischemia, 1 hour reperfusion, and a single dose of EPO; and group V (erythropoietin 2, EPO2 ), 3 hours ischemia, 48 hours reperfusion, and 2 doses of EPO. Malondialdehyde (MDA) and nitric oxide (NO) levels and activities of superoxide dismutase and catalase were measured. Tissue damage to ovarian tissue was scored by histologic examination. Data were compared among groups with parametric tests. Results The MDA levels in the S and EPO groups were significantly lower than the T/D groups ( P < .001). Catalase and superoxide dismutase activities, and NO levels in the S and EPO groups were significantly higher than in the T/D groups ( P < .05). Ovarian tissue damage in the S and EPO groups was significantly less than in the T/D groups ( P < .05). Levels of all biochemical markers and ovarian tissue damage scores were similar among the S, EPO1 , and EPO2 groups ( P > .05). Conclusion Erythropoietin attenuates ischemia-reperfusion injury when given during the acute phase of ovarian torsion-detorsion in a rat model.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19853760</pmid><doi>10.1016/j.jpedsurg.2009.02.071</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Antioxidants - therapeutic use Catalase - metabolism Disease Models, Animal Erythropoietin Erythropoietin - therapeutic use Female Ischemia-reperfusion Malondialdehyde - metabolism Nitric Oxide - metabolism Ovarian Diseases - metabolism Ovarian Diseases - pathology Ovarian Diseases - prevention & control Ovarian torsion-detorsion Ovary - metabolism Ovary - pathology Pediatrics Random Allocation Rats Rats, Sprague-Dawley Recombinant Proteins Reperfusion Injury - metabolism Reperfusion Injury - pathology Reperfusion Injury - prevention & control Superoxide Dismutase - metabolism Surgery Torsion Abnormality - drug therapy Torsion Abnormality - metabolism Torsion Abnormality - pathology Torsion Abnormality - prevention & control |
| title | Protective effect of erythropoietin on torsion/detorsion injury in rat model |
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