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Protective effect of erythropoietin on torsion/detorsion injury in rat model
Abstract Purpose The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats. Methods Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D1 ), 3 hours ischemia and 1 hour reperfusion; group...
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Published in: | Journal of pediatric surgery 2009-10, Vol.44 (10), p.1988-1994 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Abstract Purpose The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats. Methods Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D1 ), 3 hours ischemia and 1 hour reperfusion; group III (torsion/detorsion 2, T/D2 ), 3 hours ischemia and 48 hours reperfusion; group IV (erythropoietin 1, EPO1 ), 3 hours ischemia, 1 hour reperfusion, and a single dose of EPO; and group V (erythropoietin 2, EPO2 ), 3 hours ischemia, 48 hours reperfusion, and 2 doses of EPO. Malondialdehyde (MDA) and nitric oxide (NO) levels and activities of superoxide dismutase and catalase were measured. Tissue damage to ovarian tissue was scored by histologic examination. Data were compared among groups with parametric tests. Results The MDA levels in the S and EPO groups were significantly lower than the T/D groups ( P .05). Conclusion Erythropoietin attenuates ischemia-reperfusion injury when given during the acute phase of ovarian torsion-detorsion in a rat model. |
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ISSN: | 0022-3468 1531-5037 |
DOI: | 10.1016/j.jpedsurg.2009.02.071 |