Protective effect of erythropoietin on torsion/detorsion injury in rat model

Abstract Purpose The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats. Methods Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D1 ), 3 hours ischemia and 1 hour reperfusion; group...

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Published in:Journal of pediatric surgery 2009-10, Vol.44 (10), p.1988-1994
Main Authors: Bakan, Vedat, Çıralık, Harun, Tolun, Fatma İnanaç, Atlı, Yalçın, Mil, Ayhan, Öztürk, Şenol
Format: Article
Language:English
Subjects:
Animals
Antioxidants - therapeutic use
Catalase - metabolism
Disease Models, Animal
Erythropoietin
Erythropoietin - therapeutic use
Female
Ischemia-reperfusion
Malondialdehyde - metabolism
Nitric Oxide - metabolism
Ovarian Diseases - metabolism
Ovarian Diseases - pathology
Ovarian Diseases - prevention & control
Ovarian torsion-detorsion
Ovary - metabolism
Ovary - pathology
Pediatrics
Random Allocation
Rats
Rats, Sprague-Dawley
Recombinant Proteins
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
Superoxide Dismutase - metabolism
Surgery
Torsion Abnormality - drug therapy
Torsion Abnormality - metabolism
Torsion Abnormality - pathology
Torsion Abnormality - prevention & control
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Summary:Abstract Purpose The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats. Methods Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D1 ), 3 hours ischemia and 1 hour reperfusion; group III (torsion/detorsion 2, T/D2 ), 3 hours ischemia and 48 hours reperfusion; group IV (erythropoietin 1, EPO1 ), 3 hours ischemia, 1 hour reperfusion, and a single dose of EPO; and group V (erythropoietin 2, EPO2 ), 3 hours ischemia, 48 hours reperfusion, and 2 doses of EPO. Malondialdehyde (MDA) and nitric oxide (NO) levels and activities of superoxide dismutase and catalase were measured. Tissue damage to ovarian tissue was scored by histologic examination. Data were compared among groups with parametric tests. Results The MDA levels in the S and EPO groups were significantly lower than the T/D groups ( P   .05). Conclusion Erythropoietin attenuates ischemia-reperfusion injury when given during the acute phase of ovarian torsion-detorsion in a rat model.
ISSN:0022-3468
1531-5037
DOI:10.1016/j.jpedsurg.2009.02.071