Functional analysis of putative genes encoding the PIP2 water channel subfamily in Populus trichocarpa

We located fully sequenced putative genes of the plasma membrane intrinsic proteins (PIPs) family in the Populus trichocarpa (Torr. Gray), genome. Of 23 gene candidates, we assigned eight genes to the PIP2 subfamily. All eight putative genes were expressed in vegetative tissues (roots, leaves, bark...

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Bibliographic Details
Published in:Tree physiology 2009-11, Vol.29 (11), p.1467-1477
Main Authors: Secchi, Francesca, Maciver, Bryce, Zeidel, Mark L, Zwieniecki, Maciej A
Format: Article
Language:English
Subjects:
Animals
aquaporins
Aquaporins - genetics
Aquaporins - metabolism
Aquaporins - physiology
Biological Transport - genetics
forest trees
gene expression
Gene Expression - drug effects
genes
genomics
heavy metal inhibition
heavy metals
Hydrogen-Ion Concentration
intrinsic proteins
ions
mercury
Metals, Heavy - pharmacology
Multigene Family - physiology
oocytes
Phylogeny
plant proteins
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Proteins - physiology
plant tissues
plant-water relations
polymerase chain reaction
Populus - drug effects
Populus - genetics
Populus - metabolism
Populus balsamifera subsp. trichocarpa
sequence analysis
Water - metabolism
Xenopus
Xenopus laevis
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Summary:We located fully sequenced putative genes of the plasma membrane intrinsic proteins (PIPs) family in the Populus trichocarpa (Torr. Gray), genome. Of 23 gene candidates, we assigned eight genes to the PIP2 subfamily. All eight putative genes were expressed in vegetative tissues (roots, leaves, bark and wood), and all of them showed water channel activity after being expressed in Xenopus oocytes. Six of eight proteins were affected by mercury ions. No proteins were affected by the presence of nickel or tungsten ions, or by lowering the pH of bathing external solution from 7.4 to 6.5. The presence of copper ions caused seven of eight PIP2 proteins to increase their water transport capacity by as much as 50%. This systematic study of the PIP2 subfamily of proteins in P. trichocarpa provides a basic overview of their activity as water channels and will be a useful reference for future physiological studies of plant water relations that use P. trichocarpa as a model system.
ISSN:0829-318X
1758-4469
DOI:10.1093/treephys/tpp060