The Receptor-Like Cytoplasmic Kinase (OsRLCK) Gene Family in Rice: Organization, Phylogenetic Relationship, and Expression during Development and Stress

Receptor-like cytoplasmic kinases (RLCKs) in plants belong to the super family of receptor-like kinases (RLKs). These proteins show homology to RLKs in kinase domain but lack the transmembrane domain. Some of the functionally characterized RLCKs from plants have been shown to play roles in developme...

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Published in:	Molecular plant 2008-09, Vol.1 (5), p.732-750
Main Authors:	Vij, Shubha, Giri, Jitender, Dansana, Prasant Kumar, Kapoor, Sanjay, Tyagi, Akhilesh K.
Format:	Article
Language:	eng
Subjects:	abiotic stress biotic stress Chromosomes, Plant - genetics Cytoplasm - enzymology Gene Duplication Gene Expression Profiling Gene Expression Regulation, Plant Genome, Plant - genetics genome-wide analysis kinase Multigene Family - genetics Oryza - enzymology Oryza - genetics Oryza - growth & development Oryza - physiology Phylogeny Protein Structure, Tertiary Receptor Protein-Tyrosine Kinases - chemistry Receptor Protein-Tyrosine Kinases - genetics Reproducibility of Results rice RLCK Stress, Physiological - genetics
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Summary:	Receptor-like cytoplasmic kinases (RLCKs) in plants belong to the super family of receptor-like kinases (RLKs). These proteins show homology to RLKs in kinase domain but lack the transmembrane domain. Some of the functionally characterized RLCKs from plants have been shown to play roles in development and stress responses. Previously, 149 and 187 RLCK encoding genes were identified from Arabidopsis and rice, respectively. By using HMM-based domain structure and phylogenetic relationships, we have identified 379 OsRLCK s from rice. OsRLCK s are distributed on all 12 chromosomes of rice and some members are located on duplicated chromosomal segments. Several OsRLCK s probably also undergo alternative splicing, some having evidence only in the form of gene models. To understand their possible functions, expression patterns during landmark stages of vegetative and reproductive development as well as abiotic and biotic stress using microarray and MPSS-based data were analyzed. Real-time PCR-based expression profiling for a selected few genes confirmed the outcome of microarray analysis. Differential expression patterns observed for majority of OsRLCK s during development and stress suggest their involvement in diverse functions in rice. Majority of the stress-responsive OsRLCK s were also found to be localized within mapped regions of abiotic stress QTLs. Outcome of this study would help in selecting organ/development stage specific OsRLCK genes/targets for functional validation studies.
ISSN:	1674-2052 1752-9867