Characterization of a microbial community capable of nitrification at cold temperature

While the oxidation of ammonia is an integral component of advanced aerobic livestock wastewater treatment, the rate of nitrification by ammonia-oxidizing bacteria is drastically reduced at colder temperatures. In this study we report an acclimated lagoon nitrifying sludge that is capable of high ra...

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Bibliographic Details
Published in:Bioresource technology 2010, Vol.101 (2), p.491-500
Main Authors: Ducey, Thomas F., Vanotti, Matias B., Shriner, Anthony D., Szogi, Ariel A., Ellison, Aprel Q.
Format: Article
Language:English
Subjects:
16S rRNA gene
Acidobacteria
Actinobacteria
Activated sludge
ammonia
Ammonia - metabolism
Ammonia-oxidizing bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - metabolism
Bacteroidetes
Base Sequence
Biological and medical sciences
Biological treatment of sewage sludges and wastes
Biotechnology
Cold Temperature
DNA Primers
DNA, Bacterial
Environment and pollution
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Manure
Nitrates - metabolism
nitrification
Nitrite
Nitrites - metabolism
Nitrosomonas
oxidation
Phylogeny
Proteobacteria
species diversity
Verrucomicrobia
Verrucomicrobiaceae
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Summary:While the oxidation of ammonia is an integral component of advanced aerobic livestock wastewater treatment, the rate of nitrification by ammonia-oxidizing bacteria is drastically reduced at colder temperatures. In this study we report an acclimated lagoon nitrifying sludge that is capable of high rates of nitrification at temperatures from 5 °C (11.2 mg N/g MLVSS/h) to 20 °C (40.4 mg N/g MLVSS/h). The composition of the microbial community present in the nitrifying sludge was investigated by partial 16S rRNA gene sequencing. After DNA extraction and the creation of a plasmid library, 153 partial length 16S rRNA gene clones were sequenced and analyzed phylogenetically. Over 80% of these clones were affiliated with the Proteobacteria, and grouped with the β- (114 clones), γ- (7 clones), and α-classes (2 clones). The remaining clones were affiliated with the Acidobacteria (1 clone), Actinobacteria (8 clones), Bacteroidetes (16 clones), and Verrucomicrobia (5 clones). The majority of the clones belonged to the genus Nitrosomonas, while other clones affiliated with microorganisms previously identified as having floc forming or psychrotolerance characteristics.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2009.07.091