Impact of Human Cytomegalovirus Infection UL55-Nested Polymerase Chain Reaction Method in Hematopoietic Stem Cell Transplant Donors and Recipients

Abstract Human cytomegalovirus (HCMV) is one of the most important and critical viral causes of graft rejection among hematopoietic stem cell transplant (HSCT) recipients. Monitoring of this viral infection has a critical role in the management of HSCT clinical complications. In this retrospective c...

Full description

Saved in:
Bibliographic Details
Published in:Transplantation proceedings 2009-09, Vol.41 (7), p.2898-2899
Main Authors: Banan, A.A, Yaghobi, R, Ramzi, M, Mehrabani, D
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cytomegalovirus - genetics
Cytomegalovirus - isolation & purification
Cytomegalovirus Infections - blood
Cytomegalovirus Infections - diagnosis
Cytomegalovirus Infections - epidemiology
Cytomegalovirus Infections - genetics
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Genotype
Hematopoietic Stem Cell Transplantation - adverse effects
Humans
Infectious diseases
Medical sciences
Polymerase Chain Reaction - methods
Surgery
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Tissue Donors - statistics & numerical data
Tissue, organ and graft immunology
Viral diseases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Human cytomegalovirus (HCMV) is one of the most important and critical viral causes of graft rejection among hematopoietic stem cell transplant (HSCT) recipients. Monitoring of this viral infection has a critical role in the management of HSCT clinical complications. In this retrospective cohort, blood (plasma and buffy coat) and urine samples were collected from 110 HSCT patients and 95 donors pretransplantation and weekly for 100 days posttransplantation. An HCMV-optimized UL55-nested polymerase chain reaction (PCR) method was used to detect HCMV infection. Genotyping of the HCMV UL55 gene was performed for all UL55-nested, PCR-positive samples. HSCT donor and recipient laboratory and clinical data were statistically analyzed using SPSS version 15 software. UL55-nested, PCR-positive results were obtained in 3540/4950 (71.5%), 3634/4950 (73.4%), and 3292/4950 (66.5%) of these plasma, buffy coat, and urine samples, respectively. Twenty-five percent of transplant donors were infected with HCMV. An increase in HCMV infection was observed from pre- to post-HSCT conditions. Detection of the gB2 UL55 genotype in most transplant patient samples suggested the need to examine the possible impact of HCMV UL55 genotypes and HCMV infections among stem cell transplant recipients.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2009.07.042