Identification of CD3+ T lymphocytes in the green turtle Chelonia mydas

To understand the role of the immune system with respect to disease in reptiles, there is the need to develop tools to assess the host's immune response. An important tool is the development of molecular markers to identify immune cells, and these are limited for reptiles. We developed a techni...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2009-10, Vol.131 (3), p.211-217
Main Authors: Muñoz, Fernando A., Estrada-Parra, Sergio, Romero-Rojas, Andres, Work, Thierry M., Gonzalez-Ballesteros, Erik, Estrada-Garcia, Iris
Format: Article
Language:English
Subjects:
Adaptive Immunity
Animals
Blotting, Western
CD3 Complex - metabolism
Cell Separation - methods
Cell Separation - veterinary
Chelonia mydas
Cryopreservation - methods
Cryopreservation - veterinary
Cryopreservation of mononuclear cells
Delayed-type hypersensitivity
Flow Cytometry
Immunohistochemistry
Immunophenotyping
Lymphoid Tissue - cytology
Lymphoid Tissue - immunology
Marine
Phytohemagglutinins - pharmacology
Reptilian T lymphocyte
Skin - cytology
Skin - immunology
T-Lymphocyte Subsets - cytology
T-Lymphocyte Subsets - immunology
TCR/CD3 complex
Turtles - blood
Turtles - immunology
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Summary:To understand the role of the immune system with respect to disease in reptiles, there is the need to develop tools to assess the host's immune response. An important tool is the development of molecular markers to identify immune cells, and these are limited for reptiles. We developed a technique for the cryopreservation of peripheral blood mononuclear cells and showed that a commercially available anti-CD3 epsilon chain antibody detects a subpopulation of CD3 positive peripheral blood lymphocytes in the marine turtle Chelonia mydas. In the thymus and in skin inoculated with phytohemagglutinin, the same antibody showed the classical staining pattern observed in mammals and birds. For Western blot, the anti-CD3 antibodies identified a 17.6 kDa band in membrane proteins of peripheral blood mononuclear cell compatible in weight to previously described CD3 molecules. This is the first demostration of CD3+ cells in reptiles using specific antibodies.
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2009.04.015