Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

Realizing the full potential of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) requires efficient methods for genetic modification. However, techniques to generate cell type-specific lineage reporters, as well as reliable tools to disrupt, repair or overexpress genes...

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Bibliographic Details

Published in:	Nature biotechnology 2009-09, Vol.27 (9), p.851-857
Main Authors:	Amora, Ranier, Miller, Jeffrey C, Mitalipova, Maisam, Katibah, George E, Rebar, Edward J, Urnov, Fyodor D, Jaenisch, Rudolf, Gregory, Philip D, Boydston, Elizabeth A, Beard, Caroline, Hockemeyer, Dirk, Zeitler, Bryan, Meng, Xiangdong, Soldner, Frank, Gao, Qing, Zhang, Lei, DeKelver, Russell C
Format:	Article
Language:	eng
Subjects:	Biological and medical sciences Biotechnology Cell Line Deoxyribonucleases - genetics Deoxyribonucleases - metabolism Embryonic stem cells Embryonic Stem Cells - physiology Fundamental and applied biological sciences. Psychology Gene Expression Gene Silencing Gene targeting Gene Targeting - methods Genetic aspects Genetic engineering Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Immunohistochemistry Nucleases Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism Physiological aspects Pluripotent Stem Cells - physiology Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Stem cells Transcription Factors - genetics Transcription Factors - metabolism Zinc Zinc finger proteins Zinc Fingers - physiology
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