Isolation, purification and antibacterial effects of fusaproliferin produced by Fusariumsubglutinans in submerged culture

To evaluate the fusaproliferin (FUS) production, Fusariumsubglutinans ITEM 2404 was grown in a liquid medium of potato being this mycotoxin purified by high-performance liquid chromatography (HPLC) with a C18 semipreparative column using a mobile phase of acetonitrile/H(2)O using gradient conditions...

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Bibliographic Details
Published in:Food and chemical toxicology 2009-10, Vol.47 (10), p.2539-2543
Main Authors: Meca, G, Sospedra, I, Soriano, J M, Ritieni, A, Valero, M A, Mañes, J
Format: Article
Language:English
Subjects:
Absorbance
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - isolation & purification
Anti-Bacterial Agents - pharmacology
Biological
Chromatography, High Pressure Liquid
Culture
Escherichia coli - drug effects
Escherichia coli - growth & development
Fusarium - chemistry
Fusarium - physiology
Liquid chromatography
Liquids
Magnetic Resonance Spectroscopy
Microorganisms
Mycotoxins
Spectrometry, Mass, Electrospray Ionization
Staphylococcus aureus - drug effects
Staphylococcus aureus - growth & development
Terpenes - analysis
Terpenes - isolation & purification
Terpenes - pharmacology
Ultraviolet
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Summary:To evaluate the fusaproliferin (FUS) production, Fusariumsubglutinans ITEM 2404 was grown in a liquid medium of potato being this mycotoxin purified by high-performance liquid chromatography (HPLC) with a C18 semipreparative column using a mobile phase of acetonitrile/H(2)O using gradient conditions. The purity of the fusaproliferin was verified by analytical HPLC, ultraviolet absorbance measurements, LC/MS-MS, (1)H NMR spectroscopy. The isolated FUS was shown to be free of impurities and can be used as a standard for routine analysis. The pure fusaproliferin was utilized to study the biological activity on Escherichiacoli and Staphylococcusaureus. This study demostred that FUS not showed significant antimicrobial activity against these microorganisms.
ISSN:0278-6915
1873-6351
DOI:10.1016/j.fct.2009.07.014