Thiamin Confers Enhanced Tolerance to Oxidative Stress in Arabidopsis

Thiamin and thiamin pyrophosphate (TPP) are well known for their important roles in human nutrition and enzyme catalysis. In this work, we present new evidence for an additional role of these compounds in the protection of cells against oxidative damage. Arabidopsis (Arabidopsis thaliana) plants sub...

Full description

Saved in:

Bibliographic Details
Published in:	Plant physiology (Bethesda) 2009-09, Vol.151 (1), p.421-432
Main Authors:	Tunc-Ozdemir, Meral, Miller, Gad, Song, Luhua, Kim, James, Sodek, Ahmet, Koussevitzky, Shai, Misra, Amarendra Narayan, Mittler, Ron, Shintani, David
Format:	Article
Language:	eng
Subjects:	Antioxidants Arabidopsis - drug effects Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Biological and medical sciences Biosynthesis Development and progression Environmental Stress and Adaptation to Stress Enzymes Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant - physiology Hardiness Mutation Observations Oxidation-Reduction Oxidative stress Oxidative Stress - physiology Phosphates Physiological aspects Plant physiology and development Plants Properties Reactive Oxygen Species Root growth Salicylic Acid - metabolism Seedlings Seedlings - physiology Stress tests T tests Thiamine - pharmacology Vitamin B1
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Thiamin and thiamin pyrophosphate (TPP) are well known for their important roles in human nutrition and enzyme catalysis. In this work, we present new evidence for an additional role of these compounds in the protection of cells against oxidative damage. Arabidopsis (Arabidopsis thaliana) plants subjected to abiotic stress conditions, such as high light, cold, osmotic, salinity, and oxidative treatments, accumulated thiamin and TPP. Moreover, the accumulation of these compounds in plants subjected to oxidative stress was accompanied by enhanced expression of transcripts encoding thiamin biosynthetic enzymes. When supplemented with exogenous thiamin, wild-type plants displayed enhanced tolerance to oxidative stress induced by paraquat. Thiamin application was also found to protect the reactive oxygen species-sensitive ascorbate peroxidase1 mutant from oxidative stress. Thiamin-induced tolerance to oxidative stress was accompanied by decreased production of reactive oxygen species in plants, as evidenced from decreased protein carbonylation and hydrogen peroxide accumulation. Because thiamin could protect the salicylic acid induction-deficient1 mutant against oxidative stress, thiamin-induced oxidative protection is likely independent of salicylic acid signaling or accumulation. Taken together, our studies suggest that thiamin and TPP function as important stress-response molecules that alleviate oxidative stress during different abiotic stress conditions.
ISSN:	0032-0889 1532-2548 1532-2548