Antihypertensive activity of Salvia elegans Vahl. (Lamiaceae): ACE inhibition and angiotensin II antagonism

Oral administration of Salvia elegans Vahl. (a) extract and fractions significantly decreases diastolic blood pressure in mice with hypertension after AG II i.v. administration (b). Salvia elegans Vahl. (Lamiaceae), recognized with the popular name of “mirto” is widely used in Mexico for healing pur...

Full description

Saved in:
Bibliographic Details
Published in:Journal of ethnopharmacology 2010-07, Vol.130 (2), p.340-346
Main Authors: Jiménez-Ferrer, Enrique, Hernández Badillo, Fidel, González-Cortazar, Manases, Tortoriello, Jaime, Herrera-Ruiz, Maribel
Format: Article
Language:English
Subjects:
Angiotensin converting enzyme
Angiotensin II
Angiotensin II - administration & dosage
Angiotensin II - antagonists & inhibitors
Angiotensin II Type 1 Receptor Blockers - pharmacology
Angiotensin-Converting Enzyme Inhibitors - pharmacology
animal models
Animals
antagonists
antihypertensive agents
Antihypertensive Agents - pharmacology
antihypertensive effect
biochemical pathways
Biological and medical sciences
blood pressure
Blood Pressure - drug effects
Chromatography, Thin Layer
diastolic blood pressure
Disease Models, Animal
Dose-Response Relationship, Drug
enzyme inhibition
enzymes
General pharmacology
hypertension
Hypertension - chemically induced
Hypertension - physiopathology
Hypertension - prevention & control
Injections, Intravenous
Kinetics
Lisinopril - pharmacology
Losartan - pharmacology
Medical sciences
medicinal plants
Mice
Mice, Inbred ICR
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A - metabolism
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
phenylpropanoids
physiological regulation
Plant Components, Aerial
Plant Extracts - chemistry
Plant Extracts - pharmacology
Salvia
Salvia elegans
Spectrophotometry, Ultraviolet
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Oral administration of Salvia elegans Vahl. (a) extract and fractions significantly decreases diastolic blood pressure in mice with hypertension after AG II i.v. administration (b). Salvia elegans Vahl. (Lamiaceae), recognized with the popular name of “mirto” is widely used in Mexico for healing purposes, and also them as antihypertensive treatment. The high prevalence of this illness and the side effects of antihypertensive drugs conducted us to the evaluation of the Salvia elegans extract on angiotensin II action. The acute response of blood pressure to angiotensin II administration was measured in mice. We also tested in vitro the inhibitory effect on angiotensin convertase enzyme. Additionally, characterization of the pharmacological effect of the extract fraction was obtained. We obtained dose–response curve for the administration of complete extract and extract fractions. Due to the hydroalcoholic extract (SeHA) treatment blood pressure decreased significantly from systolic dose of 0.75 mg kg −1 ( p < 0.05) and even had an antihypertensive effect that was greater than that treatment with losartan. SeHA extract decreased the E max of the AG II hypertensive effect by about 20% in both systolic and diastolic pressures, treatment with losartan also decreased the same parameter between 6% and 8% for systolic and diastolic pressures, respectively. Fractions SeF8 and SeF8-8 showed similar levels of AG II ED 50 for both pressures compared with losartan, these fractions showed major compounds with maximum absorbance peaks at 221, 289 and 330 nm typical of flavonoids. In the inhibition assay the activity of angiotensin converting enzyme (ACE), the extract SeHA showed percentage inhibition (%IACE) of 50.27 ± 5.09% ( n = 5). SeBuOH fraction is found to have greater inhibitory capacity of achieving a IACE 78.40 ± 2.24% ( n = 5), which was similar to the values obtained in the presence of the SeF8-22 fraction (82.61 ± 1.74%) and lisinopril (87.18 ± 1.16%). The changes in the value of K M suggest that components of the extracts and fractions were recognized by the enzyme's active site. The main compounds of the fractions SeBuOH, SeF8-22 were by flavonoid and phenyl propanoid types, according to UV absorption spectra of the fractions. The experimental results demonstrated the antihypertensive effect of Salvia elegans and it was due to the AG II antagonism and inhibition of angiotensin converting enzyme.
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2010.05.013