Platycodin D inhibits adipogenesis of 3T3-L1 cells by modulating kruppel-like factor 2 and peroxisome proliferator-activated receptor γ

In this study, platycodin D was found to inhibit intracellular triglyceride accumulation in 3T3-L1 cells with an IC₅₀ of 7.1 μM. The expression levels of genes involved in lipid metabolism such as fatty-acid-binding protein 4 and lipoprotein lipase were significantly downregulated following treatmen...

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Published in:Phytotherapy research 2010-06, Vol.24 (S2), p.S161-S167
Main Authors: Lee, Haeyong, Kang, Ryunhwa, Kim, Yeong Shik, Chung, Sang-In, Yoon, Yoosik
Format: Article
Language:English
Subjects:
3T3-L1
3T3-L1 Cells
adipogenesis
Adipogenesis - drug effects
Animals
Gene Expression Regulation
KLF2
Kruppel-Like Transcription Factors - metabolism
Lipid Metabolism
Mice
obesity
platycodin D
PPAR gamma - metabolism
PPARγ
RNA, Messenger - metabolism
Saponins - pharmacology
Triglycerides - analysis
Triterpenes - pharmacology
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Summary:In this study, platycodin D was found to inhibit intracellular triglyceride accumulation in 3T3-L1 cells with an IC₅₀ of 7.1 μM. The expression levels of genes involved in lipid metabolism such as fatty-acid-binding protein 4 and lipoprotein lipase were significantly downregulated following treatment with platycodin D. Treatment with platycodin D also resulted in a reduction of Peroxisome proliferator-activated receptor(PPAR)γ expression and its binding to target DNA sequence. Among the various upstream regulators of PPARγ, the expression of Kruppel-like factor(KLF)2, an anti-adipogenic factor, was significantly upregulated following platycodin D treatment. When the upregulation of KLF2 was inhibited by KLF2 siRNA, the expression and binding of PPARγ to its target sequence were significantly recovered under these conditions. The results of this study suggested that anti-adipogenic effect of platycodin D involves the upregulation of KLF2 and subsequent downregulation of PPARγ. Copyright © 2009 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.3054