Real-time quantitative RT-PCR assessment of PIM-1 and hK2 mRNA expression in benign prostate hyperplasia and prostate cancer

To investigate the expressions of PIM-1 and hK2 mRNA in normal prostate, benign prostatic glandular hyperplasia (BPH), and prostate cancer (PCa), and to explore the association of PIM-1 and hK2 expressions with PCa progression. The samples were harvested from 37 patients with BPH, 23 patients with P...

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Bibliographic Details
Published in:Medical oncology (Northwood, London, England) London, England), 2009-09, Vol.26 (3), p.303-308
Main Authors: He, Hui-chan, Bi, Xue-cheng, Zheng, Zhi-wei, Dai, Qi-shan, Han, Zhao-Dong, Liang, Yu-Xiang, Ye, Yong-Kang, Zeng, Guo-hua, Zhu, Gang, Zhong, Wei-de
Format: Article
Language:English
Subjects:
Aged
Benign
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - genetics
Chi-Square Distribution
Gene Expression
Hematology
Humans
Hyperplasia
Internal Medicine
Male
Medicine
Medicine & Public Health
Oncology
Organic Chemicals - chemistry
Original Paper
Pathology
Polymerase chain reaction
Prognosis
Prostate cancer
Prostatic Hyperplasia - genetics
Prostatic Hyperplasia - metabolism
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Proto-Oncogene Proteins c-pim-1 - biosynthesis
Proto-Oncogene Proteins c-pim-1 - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Sensitivity and Specificity
Tissue Kallikreins - biosynthesis
Tissue Kallikreins - genetics
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Summary:To investigate the expressions of PIM-1 and hK2 mRNA in normal prostate, benign prostatic glandular hyperplasia (BPH), and prostate cancer (PCa), and to explore the association of PIM-1 and hK2 expressions with PCa progression. The samples were harvested from 37 patients with BPH, 23 patients with PCa, and three with normal prostate tissues. Total RNA was extracted from their prostate tissues and analyzed for PIM-1 and hK2 mRNA levels using SYBR green I-based quantitative real-time RT-PCR (QRT-PCR) assays and Southern blot analysis. The differences of gene expressions were calculated based on standard curve. Quantitative expressions of PIM-1 and hK2 mRNA in normal prostate, BPH, and PCa were 1.05 ± 0.04, 2.57 ± 0.74, 4.45 ± 0.63, and 1.02 ± 0.03, 2.264 ± 0.46, 5.905 ± 0.78, respectively. PIM-1 and hK2 were expressed higher in PCa than those in BPH and normal prostate tissues, the differences among which had statistic significance ( P  
ISSN:1357-0560
1559-131X
DOI:10.1007/s12032-008-9120-9