Intra-cervical application of Misoprostol at estrus alters the content of cervical hyaluronan and the mRNA expression of follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR) and cyclooxygenase-2 in the ewe

The complex anatomy the of ovine cervix limits the success of transcervical artificial insemination in sheep, but Misoprostol (a PGE1 analogue) relaxes the cervix and facilitates transcervical artificial insemination. However, the mechanism by which Misoprostol causes cervical relaxation is not know...

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Bibliographic Details
Published in:Theriogenology 2010-06, Vol.73 (9), p.1257-1266
Main Authors: Leethongdee, S., Kershaw-Young, C.M., Scaramuzzi, R.J., Khalid, M.
Format: Article
Language:English
Subjects:
Animals
artificial insemination
biochemical mechanisms
biosynthesis
Cervix
Cervix Uteri - chemistry
Cervix Uteri - drug effects
controlled internal drug release devices
COX-2
Cyclooxygenase 1 - genetics
Estrus
estrus synchronization
ewes
Feedback, Physiological
Female
follicle-stimulating hormone
Gene Expression
Gonadotrophin receptors
hormonal regulation
hormone receptors
Hyaluronan
hyaluronic acid
Hyaluronic Acid - analysis
In Situ Hybridization
luteinizing hormone
messenger RNA
Misoprostol
Misoprostol - administration & dosage
physiological response
prostaglandin synthase
Receptors, FSH - genetics
Receptors, Gonadotropin - genetics
Receptors, LH - genetics
RNA, Messenger - analysis
Sheep - metabolism
synthetic prostaglandins
vagina
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Summary:The complex anatomy the of ovine cervix limits the success of transcervical artificial insemination in sheep, but Misoprostol (a PGE1 analogue) relaxes the cervix and facilitates transcervical artificial insemination. However, the mechanism by which Misoprostol causes cervical relaxation is not known. This study examined if intra-cervical Misoprostol altered the hyaluronan content and the mRNA expression of COX-2, LHR, or FSHR in the cervix of the estrus ewe. Estrus was synchronized in cyclic ewes with progestagen pessaries and 48h after sponge removal ewes were treated intra-cervically with 0 (controls), 200, or 400μg Misoprostol. Hyaluronan content was determined by ELISA and mRNA expression of LHR, FSHR, and COX-2 was analyzed by in situ hybridization using digoxigenin-11-uridine-5′-triphosphate labeled riboprobes. The hyaluronan content of the cervix was significantly higher in sheep that received 200 (P
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2009.12.005