High sucrose intake in rats is associated with increased ACE2 and angiotensin-(1–7) levels in the adipose tissue

Sucrose-fed rats, a model of metabolic syndrome, are characterized by insulin resistance, obesity, hypertension, and high plasma levels of triacylglycerols and angiotensin II (Ang II). However, whether tissue renin–angiotensin system (RAS) is altered in metabolic syndrome is unclear. To study this i...

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Published in:Regulatory peptides 2010-06, Vol.162 (1), p.61-67
Main Authors: Coelho, Michella Soares, Lopes, Karen Lucasechi, Freitas, Raphael de Aquino, de Oliveira-Sales, Elizabeth Barbosa, Bergasmaschi, Cássia Toledo, Campos, Ruy Ribeiro, Casarini, Dulce Elena, Carmona, Adriana Karaoglanovic, Araújo, Mariana da Silva, Heimann, Joel Claudio, Dolnikoff, Miriam Sterman
Format: Article
Language:English
Subjects:
ACE2
Adipose Tissue - enzymology
Adipose Tissue - metabolism
Angiotensin I - metabolism
Angiotensin-(1–7)
Animals
AT 1 and AT 2 receptors
Base Sequence
Biological and medical sciences
Blood Glucose - analysis
Blotting, Western
Chromatography, High Pressure Liquid
DNA Primers
Fatty acid synthesis
Fundamental and applied biological sciences. Psychology
Male
Peptide Fragments - metabolism
Peptidyl-Dipeptidase A - metabolism
Rats
Rats, Wistar
Renin–angiotensin system
Reverse Transcriptase Polymerase Chain Reaction
Sucrose - administration & dosage
Sucrose feeding
Vertebrates: endocrinology
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Summary:Sucrose-fed rats, a model of metabolic syndrome, are characterized by insulin resistance, obesity, hypertension, and high plasma levels of triacylglycerols and angiotensin II (Ang II). However, whether tissue renin–angiotensin system (RAS) is altered in metabolic syndrome is unclear. To study this issue, food ad libitum and water (C) or 20% sucrose solution (SC) were given to adult male Wistar rats, for 30 days. Body weight (BW), blood pressure (BP), epididymal adipose tissue (EPI) mass, rate of in vivo fatty acid (FA) synthesis in EPI, circulating glucose, insulin, leptin, angiotensins I and II, triacylglycerols, and plasma renin (PRA) and angiotensin-converting enzyme (ACE) activities were evaluated. In kidneys and EPI, gene and protein expression of type 1 (AT 1) and 2 (AT 2) Ang II receptors, ACE, angiotensinogen (AGT) as well as protein expression of angiotensin-converting enzyme 2 (ACE2) were determined. In both tissues, Ang I, Ang II and Ang-(1–7) contents were also measured by HPLC. In SC rats higher BP, EPI mass, circulating triacylglycerols, insulin, leptin, PRA and, Ang II were found. In EPI, the rate of in vivo FA synthesis was associated with increased Ang-(1–7), protein expression of AT 1 and AT 2 receptors, ACE2, AGT, and gene expression of AGT although a reduction in ACE activity and in adipose Ang I and Ang II contents was observed. In kidneys, AT 1 and AT 2, ACE and AGT gene and protein expression as well as protein expression of ACE2 were unaltered while Ang II, Ang-(1–7) and ACE activity increased. These RAS component changes seem to be tissue specific and possibly are related to enhancement of FA synthesis, EPI mass and hypertension.
ISSN:0167-0115
1873-1686
DOI:10.1016/j.regpep.2010.03.008