In Vitro Assessment of Antibacterial Activity and Cytocompatibility of Silver-Containing PHBV Nanofibrous Scaffolds for Tissue Engineering

Infections with bacteria have become a serious problem in joint arthroplasty. This study reports about in vitro antibacterial activity and in vitro cell compatibility of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers loaded with metallic silver particles of a size of 5−13 nm. In vit...

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Bibliographic Details
Published in:Biomacromolecules 2010-05, Vol.11 (5), p.1248-1253
Main Authors: Xing, Zhi-Cai, Chae, Won-Pyo, Baek, Jin-Young, Choi, Moon-Jeong, Jung, Yongsoo, Kang, Inn-Kyu
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Applied sciences
Biocompatible Materials
Biological and medical sciences
Composites
Exact sciences and technology
Forms of application and semi-finished materials
Klebsiella pneumoniae - drug effects
Medical sciences
Mice
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Nanostructures
NIH 3T3 Cells
Polymer industry, paints, wood
Silver - chemistry
Staphylococcus aureus - drug effects
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology of polymers
Technology. Biomaterials. Equipments
Tissue Engineering
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Summary:Infections with bacteria have become a serious problem in joint arthroplasty. This study reports about in vitro antibacterial activity and in vitro cell compatibility of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers loaded with metallic silver particles of a size of 5−13 nm. In vitro antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae was studied by microplate proliferation tests. The adhesion, viability, and proliferation properties of fibroblasts (NIH 3T3) and differentiation of osteoblasts (MC3T3-E1) were done to study in vitro cell compatibility of the scaffolds. As the results, only silver-containing PHBV nanofibrous scaffolds showed a high antibacterial activity and an inhibitory effect on the growth of both Staphylococcus aureus and Klebsiella pneumoniae bacteria. The nanofibrous scaffolds having silver nanoparticles
ISSN:1525-7797
1526-4602
DOI:10.1021/bm1000372