Cystic fibrosis transmembrane conductance regulator and Na+ channel subunits mRNA transcripts, and Cl− efflux, show a different distribution in rat duodenum and colon

Aim:  We compared the distribution and putative association of Cl– channel transport, CFTR mRNA transcripts, and Na+ channel (ENaC)α‐ and β‐subunit mRNA transcripts in villus and crypt epithelial cells of duodenum, with corresponding surface and crypt cells of colon from sodium‐depleted rats. Method...

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Bibliographic Details
Published in:Acta physiologica Scandinavica 2003-07, Vol.178 (3), p.231-240
Main Authors: Odes, H. S., Smirnoff, P., Guberman, R., Pollak-Charcon, S., Sperber, A. D., Fich, A., Fraser, G. M., Schwartz, B.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological Transport - physiology
Chloride Channels - analysis
Cl
Colforsin - pharmacology
Colon - cytology
Colon - drug effects
Colon - metabolism
Cyclic AMP - biosynthesis
cystic fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator - analysis
Duodenum - cytology
Duodenum - drug effects
Duodenum - metabolism
Epithelium - drug effects
Epithelium - metabolism
Fundamental and applied biological sciences. Psychology
intestine
Male
Na+
Rats
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - analysis
Sodium Channels - analysis
transmembrane conductance
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Summary:Aim:  We compared the distribution and putative association of Cl– channel transport, CFTR mRNA transcripts, and Na+ channel (ENaC)α‐ and β‐subunit mRNA transcripts in villus and crypt epithelial cells of duodenum, with corresponding surface and crypt cells of colon from sodium‐depleted rats. Methods:  Cells were loaded with 36Cl– and forskolin‐stimulated efflux was determined. RT‐PCR was performed for CFTR mRNA transcripts and ENaC α‐ and β‐subunit mRNA. Duodenal epithelial cell response to VIP was assessed by measuring intracellular cAMP. Results:  Forskolin‐stimulated Cl− efflux occurred with decreasing magnitude in duodenal crypt, duodenal villus, colonic crypt and colonic surface cells in Na+‐depleted animals. CFTR expression was correlated directly with Cl− efflux (r = 0.91, P 
ISSN:0001-6772
1365-201X
DOI:10.1046/j.1365-201X.2003.01138.x