In vitro effects of phosphatidylcholine and transgalactooligosaccharides on the production of 1,2-sn-diacylglycerol by Bifidobacterium longum biovar infantis

To investigate the production of the tumour promoter 1,2-sn-diacylglycerol (DAG) by a human gut isolate of Bifidobacterium longum biovar infantis. Bifidobacterium longum biovar infantis was grown in vitro using anaerobic static batch cultures in the presence of phosphatidylcholine (PC) and trans-gal...

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Bibliographic Details
Published in:Journal of applied microbiology 2008-11, Vol.105 (5), p.1678-1685
Main Authors: Vulevic, J, Gibson, G.R
Format: Article
Language:English
Subjects:
Acetic Acid - metabolism
bifidobacteria
Bifidobacterium - growth & development
Bifidobacterium - metabolism
Bifidobacterium longum
Biological and medical sciences
Chromatography, High Pressure Liquid
Colony Count, Microbial
diacylglycerol
Diglycerides - metabolism
Feces - microbiology
Fermentation
Fundamental and applied biological sciences. Psychology
Humans
Hydrogen-Ion Concentration
Lactic Acid - metabolism
lipid metabolism
Microbiology
Oligosaccharides - metabolism
phosphatidylcholine
Phosphatidylcholines - metabolism
prebiotics
tumour promotion
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Summary:To investigate the production of the tumour promoter 1,2-sn-diacylglycerol (DAG) by a human gut isolate of Bifidobacterium longum biovar infantis. Bifidobacterium longum biovar infantis was grown in vitro using anaerobic static batch cultures in the presence of phosphatidylcholine (PC) and trans-galactooligosaccharides (TOS). Production of DAG was found to be dependent upon the presence of PC, while TOS had a reducing effect. Considerable differences in morphology, growth and metabolic end products from the micro-organism were observed under the different culture conditions. Our results have provided evidence that B. longum biovar infantis can produce DAG in vitro and that a prebiotic exerted a reducing effect upon this production. The results presented in this study demonstrate an ability of ostensibly beneficial member of the colonic environment to produce unwanted compounds under certain conditions. Therefore, it may be important that a combination of substrates and other factors are assessed when studying the behaviour of any bacterial group or species, especially when designing the dietary interventions.
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2008.03886.x