Reduction of photosystem I reaction center by recombinant DrgA protein in isolated thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803

To study the function of soluble NAD(P)H:quinone oxidoreductase of the cyanobacterium Synechocystis sp. PCC 6803 encoded by drgA gene, recombinant DrgA protein carrying 12 histidine residues on the C-terminal end was expressed in Escherichia coli and purified. Recombinant DrgA is a flavoprotein that...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2009-10, Vol.74 (10), p.1080-1087
Main Authors: Elanskaya, I. V, Toporova, V. A, Grivennikova, V. G, Muronets, E. M, Lukashev, E. P, Timofeev, K. N
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteria
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Cyanobacteria
Cyanobacteria - classification
Electron transfer reactions
Electron Transport
Electron Transport Complex I - chemistry
Escherichia coli
Life Sciences
Microbiology
Mutagenesis, Site-Directed
NAD(P)H Dehydrogenase (Quinone) - genetics
Nitroreductases - metabolism
Oxidation-Reduction
Photosynthesis
Photosystem I Protein Complex - metabolism
Quinone Reductases - metabolism
Recombinant Proteins - metabolism
Spectrum analysis
Synechocystis
Synechocystis - chemistry
Synechocystis - metabolism
Thylakoids - enzymology
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Summary:To study the function of soluble NAD(P)H:quinone oxidoreductase of the cyanobacterium Synechocystis sp. PCC 6803 encoded by drgA gene, recombinant DrgA protein carrying 12 histidine residues on the C-terminal end was expressed in Escherichia coli and purified. Recombinant DrgA is a flavoprotein that exhibits quinone reductase and nitroreductase activities with NAD(P)H as the electron donor. Using EPR spectroscopy, it was demonstrated that addition of recombinant DrgA protein and NADPH to DCMU-treated isolated thylakoid membranes of the cyanobacterium increased the dark rereduction rate of the photosystem I reaction center (P700⁺). Thus, DrgA can participate in electron transfer from NADPH to the electron transport chain of the Synechocystis sp. PCC 6803 thylakoid membrane.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297909100034