Metallo-β-lactamase-producing Pseudomonas putida as a reservoir of multidrug resistance elements that can be transferred to successful Pseudomonas aeruginosa clones

Objectives To study the prevalence, nature, involved genetic elements and epidemiology of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa and Pseudomonas putida isolated in a Spanish hospital between 2005 and 2008. Methods Etests were used for susceptibility testing and screening for MBLs...

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Published in:	Journal of antimicrobial chemotherapy 2010-03, Vol.65 (3), p.474-478
Main Authors:	Juan, Carlos, Zamorano, Laura, Mena, Ana, Albertí, Sebastián, Pérez, Jose Luis, Oliver, Antonio
Format:	Article
Language:	eng
Subjects:	Antibiotics. Antiinfectious agents. Antiparasitic agents Bacterial Typing Techniques beta-Lactamases - biosynthesis beta-Lactamases - genetics Biological and medical sciences blaVIM Blotting, Southern Cluster Analysis Cross Infection - epidemiology Cross Infection - microbiology DNA, Bacterial - chemistry DNA, Bacterial - genetics Drug Resistance, Multiple, Bacterial Electrophoresis, Gel, Pulsed-Field Gene Transfer, Horizontal Genotype Hospitals Humans integrons MBLs Medical sciences Microbial Sensitivity Tests Molecular Epidemiology Molecular Sequence Data Pharmacology. Drug treatments Plasmids Polymorphism, Genetic Pseudomonas aeruginosa - classification Pseudomonas aeruginosa - enzymology Pseudomonas aeruginosa - genetics Pseudomonas Infections - epidemiology Pseudomonas Infections - microbiology Pseudomonas putida - classification Pseudomonas putida - enzymology Pseudomonas putida - genetics Pseudomonas spp Restriction Mapping Sequence Analysis, DNA Spain - epidemiology
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Summary:	Objectives To study the prevalence, nature, involved genetic elements and epidemiology of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa and Pseudomonas putida isolated in a Spanish hospital between 2005 and 2008. Methods Etests were used for susceptibility testing and screening for MBLs, confirmed through blaVIM PCRs and sequencing. Clonal relatedness was evaluated by PFGE and multilocus sequence typing (MLST). MBL-carrying plasmids were characterized by restriction fragment length polymorphism, Southern blot and electroporation. MBL genetic elements were studied by cloning and sequencing. Results MBL-producing P. putida was detected in eight patients (one clone each; two harbouring blaVIM-1 and six harbouring blaVIM-2), representing 14% of all the infections by the P. putida/fluorescens group. MBLs were detected in only 0.3% of P. aeruginosa infections (11 patients) during the same period. PFGE revealed four P. aeruginosa clones: one producing blaVIM-13 (two patients); and three producing blaVIM-2 (two patients, six patients and one patient, respectively). MLST indicated that the VIM-13 clone was the internationally spread sequence type (ST)235, while the major VIM-2 lineage corresponded to ST179, which is associated with chronic respiratory infections. The VIM-1 integron was shown to have both plasmid and chromosomal location, while the VIM-13 integron was only chromosomal. The VIM-2 integron was located in the same transposon (Tn402/Tn5053-like) in all P. aeruginosa and P. putida isolates, suggesting its crucial role in the dissemination of VIM-2. Conclusions The high diversity and proportion of MBL-positive P. putida suggests an environmental reservoir of these resistance determinants. Dissemination of these multidrug resistance elements to successful P. aeruginosa clones presents a major epidemiological and clinical threat.
ISSN:	0305-7453 1460-2091