Biosynthesis of Liposidomycin-like A-90289 Antibiotics Featuring a New Type of Sulfotransferase

Lip reading: The biosynthetic gene cluster for A‐90289, a fatty acid nucleoside antibiotic, was cloned and sequenced. The sulfotransferase LipB is demonstrated to be essential for A‐90289 biosynthesis, and in vitro characterization revealed LipB utilizes p‐nitrophenylsulfate as an aryl sulfate donor...

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Published in:Chembiochem : a European journal of chemical biology 2010-01, Vol.11 (2), p.184-190
Main Authors: Funabashi, Masanori, Baba, Satoshi, Nonaka, Koichi, Hosobuchi, Masahiko, Fujita, Yoko, Shibata, Tomoyuki, Van Lanen, Steven G
Format: Article
Language:English
Subjects:
Aminoglycosides - biosynthesis
Aminoglycosides - chemistry
Anti-Bacterial Agents - biosynthesis
Anti-Bacterial Agents - chemistry
antibiotics
Azepines - chemistry
bacterial translocase I
biosynthesis
Multigene Family
Mutation
nucleosides
Streptomyces - enzymology
Streptomyces - genetics
sulfotransferase
Sulfotransferases - genetics
Sulfotransferases - metabolism
Uracil - analogs & derivatives
Uracil - biosynthesis
Uracil - chemistry
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Summary:Lip reading: The biosynthetic gene cluster for A‐90289, a fatty acid nucleoside antibiotic, was cloned and sequenced. The sulfotransferase LipB is demonstrated to be essential for A‐90289 biosynthesis, and in vitro characterization revealed LipB utilizes p‐nitrophenylsulfate as an aryl sulfate donor and a variety of nonaryl acceptors including caprazamycin A to give the 2′‐O‐sulfated product, A‐90289A.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200900665