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Amperometric monitoring of lactate accumulation in rabbit ischemic myocardium
Fabrication and characterization of miniature, flexible, planar biosensors for monitoring l-lactate accumulation in an ischemic myocardium are described. Three configurations of Au-based electrodes were fabricated by a photolithographic technique on flexible polyimide Kapton ® foil. All sensors are...
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| Published in: | Talanta (Oxford) 1997-09, Vol.44 (9), p.1527-1541 |
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| Main Authors: | , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c391t-2788ba97774c243961ce25367d5017a748c705b0925f00c1ebafd35be8251e523 |
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| container_end_page | 1541 |
| container_issue | 9 |
| container_start_page | 1527 |
| container_title | Talanta (Oxford) |
| container_volume | 44 |
| creator | Marzouk, Sayed A.M. Cosofret, Vasile V. Buck, Richard P. Yang, Hua Cascio, Wayne E. Hassan, Saad S.M. |
| description | Fabrication and characterization of miniature, flexible, planar biosensors for monitoring
l-lactate accumulation in an ischemic myocardium are described. Three configurations of Au-based electrodes were fabricated by a photolithographic technique on flexible polyimide Kapton
® foil. All sensors are based on an immobilized lactate oxidase with amperometric detection of the enzymatically produced hydrogen peroxide at a platinum-electroplated-gold base electrode polarized at 0.5 V versus Ag/AgCl. An inner electropolymeric layer is used to prevent electrode fouling and to reject the interference effects of easily oxidizable molecules. In addition, a diffusion controlling outer layer that greatly enhances the linear dynamic range of the sensor, is obtained by casting a polyurethane external film. The developed sensor was evaluated in vitro and proved to have high selectivity, good operational stability, good accuracy and precision (average recovery = 102.3 ± 0.4% for control sera), fast response time (
t
95 = 20 s) and high upper limit of the linear dynamic range (25–80 mM, with sensitivity of 1.7-0.4 nA mM
−1 respectively at
PO
2 = 15 mmHg). Subsequently, the sensor was brought into direct contact with the surface of the rabbit papillary muscle and used for continuous quantitative monitoring of extracellular lactate accumulation during no-flow ischemia. |
| doi_str_mv | 10.1016/S0039-9140(96)02175-3 |
| format | article |
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l-lactate accumulation in an ischemic myocardium are described. Three configurations of Au-based electrodes were fabricated by a photolithographic technique on flexible polyimide Kapton
® foil. All sensors are based on an immobilized lactate oxidase with amperometric detection of the enzymatically produced hydrogen peroxide at a platinum-electroplated-gold base electrode polarized at 0.5 V versus Ag/AgCl. An inner electropolymeric layer is used to prevent electrode fouling and to reject the interference effects of easily oxidizable molecules. In addition, a diffusion controlling outer layer that greatly enhances the linear dynamic range of the sensor, is obtained by casting a polyurethane external film. The developed sensor was evaluated in vitro and proved to have high selectivity, good operational stability, good accuracy and precision (average recovery = 102.3 ± 0.4% for control sera), fast response time (
t
95 = 20 s) and high upper limit of the linear dynamic range (25–80 mM, with sensitivity of 1.7-0.4 nA mM
−1 respectively at
PO
2 = 15 mmHg). Subsequently, the sensor was brought into direct contact with the surface of the rabbit papillary muscle and used for continuous quantitative monitoring of extracellular lactate accumulation during no-flow ischemia.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/S0039-9140(96)02175-3</identifier><identifier>PMID: 18966893</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amperometric biosensors ; Biological and medical sciences ; Biosensors ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Ischemia ; Lactate monitoring ; Methods. Procedures. Technologies ; Papillary muscle ; Various methods and equipments</subject><ispartof>Talanta (Oxford), 1997-09, Vol.44 (9), p.1527-1541</ispartof><rights>1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-2788ba97774c243961ce25367d5017a748c705b0925f00c1ebafd35be8251e523</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2787965$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18966893$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marzouk, Sayed A.M.</creatorcontrib><creatorcontrib>Cosofret, Vasile V.</creatorcontrib><creatorcontrib>Buck, Richard P.</creatorcontrib><creatorcontrib>Yang, Hua</creatorcontrib><creatorcontrib>Cascio, Wayne E.</creatorcontrib><creatorcontrib>Hassan, Saad S.M.</creatorcontrib><title>Amperometric monitoring of lactate accumulation in rabbit ischemic myocardium</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>Fabrication and characterization of miniature, flexible, planar biosensors for monitoring
l-lactate accumulation in an ischemic myocardium are described. Three configurations of Au-based electrodes were fabricated by a photolithographic technique on flexible polyimide Kapton
® foil. All sensors are based on an immobilized lactate oxidase with amperometric detection of the enzymatically produced hydrogen peroxide at a platinum-electroplated-gold base electrode polarized at 0.5 V versus Ag/AgCl. An inner electropolymeric layer is used to prevent electrode fouling and to reject the interference effects of easily oxidizable molecules. In addition, a diffusion controlling outer layer that greatly enhances the linear dynamic range of the sensor, is obtained by casting a polyurethane external film. The developed sensor was evaluated in vitro and proved to have high selectivity, good operational stability, good accuracy and precision (average recovery = 102.3 ± 0.4% for control sera), fast response time (
t
95 = 20 s) and high upper limit of the linear dynamic range (25–80 mM, with sensitivity of 1.7-0.4 nA mM
−1 respectively at
PO
2 = 15 mmHg). Subsequently, the sensor was brought into direct contact with the surface of the rabbit papillary muscle and used for continuous quantitative monitoring of extracellular lactate accumulation during no-flow ischemia.</description><subject>Amperometric biosensors</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ischemia</subject><subject>Lactate monitoring</subject><subject>Methods. Procedures. Technologies</subject><subject>Papillary muscle</subject><subject>Various methods and equipments</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkMFu1DAQhi0EotvCI4ByQAIOKePMOo5PqKooRWrFAThbzmQCRnG82A5S355sd1WOPc3l-2f--YR4JeFcgmw_fANAUxu5hXemfQ-N1KrGJ2IjO401Ko1PxeYBORGnOf8GgAYBn4sT2Zm27QxuxO1F2HGKgUvyVIU4-xKTn39WcawmR8UVrhzREpbJFR_nys9Vcn3vS-Uz_eKwT91FcmnwS3ghno1uyvzyOM_Ej6tP3y-v65uvn79cXtzUhEaWutFd1zujtd5Ss0XTSuJGYasHBVI7ve1Ig-rBNGoEIMm9GwdUPXeNkqwaPBNvD3t3Kf5ZOBcb1jY8TW7muGSrETsEJXEl1YGkFHNOPNpd8sGlOyvB7kXae5F2b8ma1t6LtPvc6-OFpQ88_E8dza3AmyPgMrlpTG4mnx-49UVtWrViHw8Yrzr-ek42k-eZePCJqdgh-kea_AN0G47-</recordid><startdate>19970901</startdate><enddate>19970901</enddate><creator>Marzouk, Sayed A.M.</creator><creator>Cosofret, Vasile V.</creator><creator>Buck, Richard P.</creator><creator>Yang, Hua</creator><creator>Cascio, Wayne E.</creator><creator>Hassan, Saad S.M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970901</creationdate><title>Amperometric monitoring of lactate accumulation in rabbit ischemic myocardium</title><author>Marzouk, Sayed A.M. ; Cosofret, Vasile V. ; Buck, Richard P. ; Yang, Hua ; Cascio, Wayne E. ; Hassan, Saad S.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-2788ba97774c243961ce25367d5017a748c705b0925f00c1ebafd35be8251e523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amperometric biosensors</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ischemia</topic><topic>Lactate monitoring</topic><topic>Methods. Procedures. Technologies</topic><topic>Papillary muscle</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marzouk, Sayed A.M.</creatorcontrib><creatorcontrib>Cosofret, Vasile V.</creatorcontrib><creatorcontrib>Buck, Richard P.</creatorcontrib><creatorcontrib>Yang, Hua</creatorcontrib><creatorcontrib>Cascio, Wayne E.</creatorcontrib><creatorcontrib>Hassan, Saad S.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marzouk, Sayed A.M.</au><au>Cosofret, Vasile V.</au><au>Buck, Richard P.</au><au>Yang, Hua</au><au>Cascio, Wayne E.</au><au>Hassan, Saad S.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amperometric monitoring of lactate accumulation in rabbit ischemic myocardium</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>1997-09-01</date><risdate>1997</risdate><volume>44</volume><issue>9</issue><spage>1527</spage><epage>1541</epage><pages>1527-1541</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Fabrication and characterization of miniature, flexible, planar biosensors for monitoring
l-lactate accumulation in an ischemic myocardium are described. Three configurations of Au-based electrodes were fabricated by a photolithographic technique on flexible polyimide Kapton
® foil. All sensors are based on an immobilized lactate oxidase with amperometric detection of the enzymatically produced hydrogen peroxide at a platinum-electroplated-gold base electrode polarized at 0.5 V versus Ag/AgCl. An inner electropolymeric layer is used to prevent electrode fouling and to reject the interference effects of easily oxidizable molecules. In addition, a diffusion controlling outer layer that greatly enhances the linear dynamic range of the sensor, is obtained by casting a polyurethane external film. The developed sensor was evaluated in vitro and proved to have high selectivity, good operational stability, good accuracy and precision (average recovery = 102.3 ± 0.4% for control sera), fast response time (
t
95 = 20 s) and high upper limit of the linear dynamic range (25–80 mM, with sensitivity of 1.7-0.4 nA mM
−1 respectively at
PO
2 = 15 mmHg). Subsequently, the sensor was brought into direct contact with the surface of the rabbit papillary muscle and used for continuous quantitative monitoring of extracellular lactate accumulation during no-flow ischemia.</abstract><cop>Amsterdam</cop><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>18966893</pmid><doi>10.1016/S0039-9140(96)02175-3</doi><tpages>15</tpages></addata></record> |
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| ispartof | Talanta (Oxford), 1997-09, Vol.44 (9), p.1527-1541 |
| issn | 0039-9140 1873-3573 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Amperometric biosensors Biological and medical sciences Biosensors Biotechnology Fundamental and applied biological sciences. Psychology Ischemia Lactate monitoring Methods. Procedures. Technologies Papillary muscle Various methods and equipments |
| title | Amperometric monitoring of lactate accumulation in rabbit ischemic myocardium |
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