Amperometric monitoring of lactate accumulation in rabbit ischemic myocardium

Fabrication and characterization of miniature, flexible, planar biosensors for monitoring l-lactate accumulation in an ischemic myocardium are described. Three configurations of Au-based electrodes were fabricated by a photolithographic technique on flexible polyimide Kapton ® foil. All sensors are...

Full description

Saved in:
Bibliographic Details
Published in:Talanta (Oxford) 1997-09, Vol.44 (9), p.1527-1541
Main Authors: Marzouk, Sayed A.M., Cosofret, Vasile V., Buck, Richard P., Yang, Hua, Cascio, Wayne E., Hassan, Saad S.M.
Format: Article
Language:English
Subjects:
Amperometric biosensors
Biological and medical sciences
Biosensors
Biotechnology
Fundamental and applied biological sciences. Psychology
Ischemia
Lactate monitoring
Methods. Procedures. Technologies
Papillary muscle
Various methods and equipments
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fabrication and characterization of miniature, flexible, planar biosensors for monitoring l-lactate accumulation in an ischemic myocardium are described. Three configurations of Au-based electrodes were fabricated by a photolithographic technique on flexible polyimide Kapton ® foil. All sensors are based on an immobilized lactate oxidase with amperometric detection of the enzymatically produced hydrogen peroxide at a platinum-electroplated-gold base electrode polarized at 0.5 V versus Ag/AgCl. An inner electropolymeric layer is used to prevent electrode fouling and to reject the interference effects of easily oxidizable molecules. In addition, a diffusion controlling outer layer that greatly enhances the linear dynamic range of the sensor, is obtained by casting a polyurethane external film. The developed sensor was evaluated in vitro and proved to have high selectivity, good operational stability, good accuracy and precision (average recovery = 102.3 ± 0.4% for control sera), fast response time ( t 95 = 20 s) and high upper limit of the linear dynamic range (25–80 mM, with sensitivity of 1.7-0.4 nA mM −1 respectively at PO 2 = 15 mmHg). Subsequently, the sensor was brought into direct contact with the surface of the rabbit papillary muscle and used for continuous quantitative monitoring of extracellular lactate accumulation during no-flow ischemia.
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(96)02175-3