A subunit vaccine against the adenovirus egg-drop syndrome using part of its fiber protein

In this study, the effectiveness of antibodies against the hexon, fiber or a fiber fragment of an avian adenovirus egg-drop syndrome (EDS), in neutralizing the virus was tested. The fiber protein is responsible for binding the virus to the target cell. The fiber fragment knob-s comprises the carboxy...

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Bibliographic Details
Published in:Vaccine 2003-06, Vol.21 (21), p.2761-2766
Main Authors: Fingerut, E, Gutter, B, Gallili, G, Michael, A, Pitcovski, J
Format: Article
Language:English
Subjects:
Adenoviridae Infections - immunology
Adenoviridae Infections - prevention & control
Adenoviridae Infections - veterinary
Adenovirus
Adenoviruses
Animals
Biological and medical sciences
Capsid Proteins - biosynthesis
Capsid Proteins - immunology
Deoxyribonucleic acid
Disease
DNA
DNA - genetics
Ducks
E coli
Egg Shell - pathology
Egg-drop syndrome
Eggs
Escherichia coli - metabolism
Fundamental and applied biological sciences. Psychology
Hemagglutination inhibition
Microbiology
Polypeptides
Poultry
Poultry Diseases - immunology
Poultry Diseases - prevention & control
Proteins
Recombinant Proteins - biosynthesis
Recombinant Proteins - immunology
Serum neutralization
Subunit vaccine
Vaccination
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Subunit - immunology
Viral Vaccines - immunology
Virology
Viruses
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Summary:In this study, the effectiveness of antibodies against the hexon, fiber or a fiber fragment of an avian adenovirus egg-drop syndrome (EDS), in neutralizing the virus was tested. The fiber protein is responsible for binding the virus to the target cell. The fiber fragment knob-s comprises the carboxy-terminal knob domain and 34 amino acids of the immediately adjacent shaft domain of the adenovirus fiber protein. The hexon, fiber capsid protein and knob-s were produced in E. coli and injected into chickens. Antibodies that were produced against the whole fiber protein showed some hemagglutination inhibition (HI) activity. Antibodies produced against the knob-s protein showed HI activity and serum neutralization (SN) activity similar to the positive control—whole virus vaccine. We assume that production of only part of the fiber enables the protein produced in E. coli to fold correctly. Antibodies produced against the hexon protein showed no SN activity. In summary, knob-s induced SN and HI antibodies against EDS virus at a rate similar to the whole virus and were significantly more efficient than the full-length fiber. The recombinant knob-s protein may be used as a vaccine against pathogenic adenovirus infections.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(03)00117-8