Thrombospondin-1, Vascular Endothelial Growth Factor Expression and Microvessel Density in Renal Cell Carcinoma and Their Relationship with Multifocality

Objective: To evaluate the relevance of microvessel density (MVD) and the angiogenic factors, vascular endothelial growth factor (VEGF, an important angiogenic factor in solid tumors) and thrombospondin-1 (TSP-1, a potent inhibitor of angiogenesis), to multifocality of renal cell carcinoma (RCC). Pa...

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Bibliographic Details
Published in:European urology 2003-07, Vol.44 (1), p.76-81
Main Authors: Baltacı, Sümer, Orhan, Diclehan, Göğüş, Çağatay, Filiz, Ercan, Tulunay, Özden, Göğüş, Orhan
Format: Article
Language:English
Subjects:
Adult
Aged
Angiogenesis Inducing Agents - analysis
Angiogenesis Inducing Agents - metabolism
Biomarkers, Tumor - analysis
Biopsy, Needle
Capillaries - physiology
Carcinoma, Renal Cell - mortality
Carcinoma, Renal Cell - pathology
Carcinoma, Renal Cell - surgery
Cohort Studies
Endothelial Growth Factors - analysis
Endothelial Growth Factors - metabolism
Female
Humans
Immunohistochemistry
Intercellular Signaling Peptides and Proteins - analysis
Intercellular Signaling Peptides and Proteins - metabolism
Kidney Neoplasms - mortality
Kidney Neoplasms - pathology
Kidney Neoplasms - surgery
Lymphokines - analysis
Lymphokines - metabolism
Male
Microcirculation
Middle Aged
Multifocality
Neoplasm Invasiveness - pathology
Neoplasm Staging
Probability
Prognosis
Renal cell carcinoma
Retrospective Studies
Sensitivity and Specificity
Statistics, Nonparametric
Survival Analysis
Thrombospondin 1 - analysis
Thrombospondin 1 - metabolism
Thrombospondin-1
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
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Summary:Objective: To evaluate the relevance of microvessel density (MVD) and the angiogenic factors, vascular endothelial growth factor (VEGF, an important angiogenic factor in solid tumors) and thrombospondin-1 (TSP-1, a potent inhibitor of angiogenesis), to multifocality of renal cell carcinoma (RCC). Patients and Methods: Using immunohistochemistry the expression of CD34, TSP-1 and VEGF was assessed in 38 archival tissue specimens from 19 patients with unifocal RCC and 19 with multifocal RCC. Immunostaining results for VEGF was scored for the appropriate percentage of positive tumor cells and relative immunostaining intensity (score range 0–12). Only extracellular immunoreactivity was considered positive for TSP-1 and the same method was used to score the stromal staining. The microvessel density was measured by immunohistochemical staining with anti-CD34 monoclonal antibody. Results: VEGF immunoreactivity≥1% was detectable in all unifocal and multifocal tumors. TSP-1 immunoreactivity was detected in 14 (73.7%) of 19 unifocal RCCs and in 16 (84.2%) of 19 multifocal RCC specimens ( p=0.69). There were no statistically significant differences in the immunostaining intensity, percentage of immunopositive cells and the staining scores of VEGF and TSP-1 among the two groups. Additionally, there was no difference in MVD in multifocal and unifocal tumors. Conclusion: As there is no difference in MVD count, and expression of angiogenic factors (VEGF and TSP-1) in multifocal and unifocal tumors, multifocality of RCC is not determined by VEGF/TSP-1 expression.
ISSN:0302-2838
1873-7560
DOI:10.1016/S0302-2838(03)00192-1