A DNA-based procedure for in planta detection of Fusarium oxysporum f. sp. phaseoli

ABSTRACT We have characterized strains of Fusarium oxysporum from common bean fields in Spain that were nonpathogenic on common bean, as well as F. oxysporum strains (F. oxysporum f. sp. phaseoli) pathogenic to common bean by random amplified polymorphic DNA (RAPD) analysis. We identified a RAPD mar...

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Bibliographic Details
Published in:Phytopathology 2002-03, Vol.92 (3), p.237-244
Main Authors: ALVES-SANTOS, Fernando M, RAMOS, Brisa, GARCIA-SANCHEZ, M. Asuncion, ESLAVA, Arturo P, DIAZ-MINGUEZ, José Maria
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Fusarium oxysporum
Generalities. Techniques
Microbiology
Pathology, epidemiology, host-fungus relationships. Damages, economic importance
Phytopathology. Animal pests. Plant and forest protection
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Summary:ABSTRACT We have characterized strains of Fusarium oxysporum from common bean fields in Spain that were nonpathogenic on common bean, as well as F. oxysporum strains (F. oxysporum f. sp. phaseoli) pathogenic to common bean by random amplified polymorphic DNA (RAPD) analysis. We identified a RAPD marker (RAPD 4.12) specific for the highly virulent pathogenic strains of the seven races of F. oxysporum f. sp. phaseoli. Sequence analysis of RAPD 4.12 allowed the design of oligonucleotides that amplify a 609-bp sequence characterized amplified region (SCAR) marker (SCAR-B310A280). Under controlled environmental and greenhouse conditions, detection of the pathogen by polymerase chain reaction was 100% successful in root samples of infected but still symptomless plants and in stem samples of plants with disease severity of >/=4 in the Centro Internacional de Agricultura Tropical (CIAT; Cali, Colombia) scale. The diagnostic procedure can be completed in 5 h and allows the detection of all known races of the pathogen in plant samples at early stages of the disease with no visible symptoms.
ISSN:0031-949X
1943-7684
DOI:10.1094/phyto.2002.92.3.237