Enhanced gamete interaction in the sperm penetration assay after coincubation with pentoxifylline and human follicular fluid

To evaluate the effect of pentoxifylline and heat-inactivated human follicular fluid (FF) on performance in the sperm penetration assay (SPA) as a paradigm for the effect of these agents on human sperm-egg interaction in vivo and in vitro fertilization. Semen specimens from men undergoing SPA testin...

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Bibliographic Details
Published in:Fertility and sterility 1992-12, Vol.58 (6), p.1205-1208
Main Authors: Lambert, Hovey, Steinleitner, Alex, Eisermann, Juergen, Serpa, Nurys, Cantor, Bernard
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Birth control
Cricetinae
Female
Follicular Fluid - physiology
Gynecology. Andrology. Obstetrics
Human follicular fluid
Humans
Infertility, Male - physiopathology
Life Sciences & Biomedicine
Male
male factor infertility
Medical sciences
Obstetrics & Gynecology
pentoxifylline
Pentoxifylline - pharmacology
Reproductive Biology
Science & Technology
Sperm-Ovum Interactions - drug effects
Sperm-Ovum Interactions - physiology
Spermatozoa - drug effects
Spermatozoa - physiology
Sterility. Assisted procreation
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Summary:To evaluate the effect of pentoxifylline and heat-inactivated human follicular fluid (FF) on performance in the sperm penetration assay (SPA) as a paradigm for the effect of these agents on human sperm-egg interaction in vivo and in vitro fertilization. Semen specimens from men undergoing SPA testing for evaluation of suspected male factor infertility were coincubated with neat medium or media supplemented with pentoxifylline or human FF in a nonblinded manner. Twenty male factor infertility patients. Semen specimens were preincubated with: [1] pentoxifylline 0.25mg/mL; [2] 10% human FF; [3] pentoxifylline+human FF; and [4] neat Biggers, Whitten, and Whittingham medium. Differences in the rate of penetration of zona-free hamster oocytes. Preincubation with either human FF or pentoxifylline produced a significant improvement in hamster egg penetration rates. Coincubation with a combination of human FF and pentoxifylline resulted in a significant enhancement of penetration as compared with single agent treatment. Coincubation of sperm with human FF and pentoxifylline may provide a means of enhancing sperm activity for insemination and assisted reproduction.
ISSN:0015-0282
1556-5653
DOI:10.1016/S0015-0282(16)55570-8