Turning placenta into brain: placental mesenchymal stem cells differentiate into neurons and oligodendrocytes

Objective We aimed to induce neural stem (NSC) and progenitor cells (NPC) from human placental tissues. Study Design Placental stem cells from first-trimester placental chorionic villi and term chorion were isolated. Neural differentiation was initiated with plating on collagen, retinoic acid, and/o...

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Bibliographic Details
Published in:American journal of obstetrics and gynecology 2010-03, Vol.202 (3), p.294.e1-294.e11
Main Authors: Portmann-Lanz, C. Bettina, PhD, Schoeberlein, Andreina, PhD, Portmann, Reto, PhD, Mohr, Stefan, MD, Rollini, Pierre, PhD, Sager, Ruth, Surbek, Daniel V., MD
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Differentiation
Female
Gynecology. Andrology. Obstetrics
Humans
Immunohistochemistry
Medical sciences
mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mitosis
Muscle Proteins - metabolism
neurodifferentiation
Neurons - cytology
Neurons - metabolism
Obstetrics and Gynecology
Oligodendroglia - cytology
placenta
Placenta - cytology
Pregnancy
Tubulin - metabolism
Up-Regulation
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Summary:Objective We aimed to induce neural stem (NSC) and progenitor cells (NPC) from human placental tissues. Study Design Placental stem cells from first-trimester placental chorionic villi and term chorion were isolated. Neural differentiation was initiated with plating on collagen, retinoic acid, and/or human brain-derived neurotrophic factor and epidermal and fibroblast growth factor. Differentiation into neurons, oligodendrocytes, and astrocytes was monitored by immunohistochemistry. Two-dimensional polyacrylamide gel electrophoresis, high-performance liquid chromatography, and tandem mass spectrometry were used to identify proteins involved in the differentiation. Results Differentiated cells were mostly immediately postmitotic with some more but not fully mature postmitotic neurons. Neurons had dopaminergic or serotonergic character. Some cells differentiated into predominantly immature oligodendrocytes. Upon differentiation, neuron-specific proteins were up-regulated, whereas placental proteins were reduced. Conclusion Stem cells derived from human placenta can be differentiated into neural progenitors.
ISSN:0002-9378
1097-6868
DOI:10.1016/j.ajog.2009.10.893