Development and Validation of a Direct LC-MS-MS Method to Determine the Acrolein Metabolite 3-HPMA in Urine

A new direct method using liquid chromatography-tandem mass spectrometry has been developed and validated for quantitation of 3-hydroxypropylmercapturic acid (3-HPMA) in urine. The method is fast, simple, and does not require extraction from urine. Analyte was separated on a hydrophilic interaction...

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Bibliographic Details
Published in:Journal of chromatographic science 2010-03, Vol.48 (3), p.194-199
Main Authors: Yan, Weiying, Byrd, Gary D., Brown, Buddy G., Borgerding, Michael F.
Format: Article
Language:English
Subjects:
Acetylcysteine - analogs & derivatives
Acetylcysteine - urine
Acrolein - metabolism
Acrolein - urine
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
Chromatography, Liquid - methods
Humans
Limit of Detection
Medical sciences
Tandem Mass Spectrometry - methods
Toxicology
Various organic compounds
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Summary:A new direct method using liquid chromatography-tandem mass spectrometry has been developed and validated for quantitation of 3-hydroxypropylmercapturic acid (3-HPMA) in urine. The method is fast, simple, and does not require extraction from urine. Analyte was separated on a hydrophilic interaction liquid chromatography column. Severe ion suppression was circumvented by a fast gradient after separation. Assay specificity, linearity, precision, and accuracy met the required FDA/CDER bioanalytical method criteria. Matrix effect and carryover of the assay were assessed. Urine sample storage stability and standard solution stability were also tested. The limit of quantitation was 22.0 ng/mL. The results for 3-HPMA obtained by our method were significantly correlated with results obtained by a contract lab.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/48.3.194