Loss of Neuronal Protein Expression in Mouse Hippocampus After Irradiation

The effects of radiation on neurons are incompletely characterized. We evaluated changes in the expression of neuronal nuclear and other proteins in the mouse hippocampus after 17-Gy whole-brain irradiation. Expression of neuronal nuclei (NeuN), neuron-specific enolas, prospero-related homeobox 1 (P...

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Bibliographic Details
Published in:Journal of neuropathology and experimental neurology 2010-03, Vol.69 (3), p.272-280
Main Authors: Wu, Kai-Liang, Li, Yu-Qing, Tabassum, Arshia, Lu, Wei-Yang, Aubert, Isabelle, Wong, C Shun
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Biological and medical sciences
Biomarkers - analysis
Biomarkers - metabolism
Calbindin 1
Calbindins
Cell Count
Cell Death - physiology
Cell Death - radiation effects
Chemotherapy
Disease Models, Animal
Down-Regulation - physiology
Down-Regulation - radiation effects
Hippocampus - metabolism
Hippocampus - physiopathology
Hippocampus - radiation effects
Homeodomain Proteins - metabolism
Homeodomain Proteins - radiation effects
Immunohistochemistry
Male
Medical sciences
Mice
Mice, Inbred C57BL
Nerve Degeneration - etiology
Nerve Degeneration - metabolism
Nerve Degeneration - physiopathology
Nerve Tissue Proteins - metabolism
Nerve Tissue Proteins - radiation effects
Neurology
Neurons - metabolism
Neurons - pathology
Neurons - radiation effects
Nuclear Proteins - metabolism
Nuclear Proteins - radiation effects
Pharmacology. Drug treatments
Phosphopyruvate Hydratase - metabolism
Phosphopyruvate Hydratase - radiation effects
Pyramidal Cells - metabolism
Pyramidal Cells - pathology
Pyramidal Cells - radiation effects
Radiation Injuries, Experimental - metabolism
Radiation Injuries, Experimental - physiopathology
Radiation, Ionizing
S100 Calcium Binding Protein G - metabolism
S100 Calcium Binding Protein G - radiation effects
Synaptophysin - metabolism
Synaptophysin - radiation effects
Tumor Suppressor Proteins - metabolism
Tumor Suppressor Proteins - radiation effects
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Summary:The effects of radiation on neurons are incompletely characterized. We evaluated changes in the expression of neuronal nuclear and other proteins in the mouse hippocampus after 17-Gy whole-brain irradiation. Expression of neuronal nuclei (NeuN), neuron-specific enolas, prospero-related homeobox 1 (Prox1), calbindin D28k, and synaptophysin 1 in the CA1, CA3, and dentate gyrus of the hippocampus was determined by immunohistochemistry; neuronal numbers were estimated by design-based stereology. At 7 days after irradiation, therewas a marked reduction of NeuN neurons in CA3. Stereologic estimates confirmed a significant reduction in NeuN neurons in CA3 at 7 days, in the dentate gyrus at 7 days, 3 weeks and 2 months, and in CA1 at 2 months compared with controls; neuron-specific enolase and prospero-related homeobox 1-positive neurons in the CA3 subregion were also decreased at 7 days. The numbers of granule and pyramidal cells identified by 4′6-diamidino-2-phenylindole nuclear staining, however, remained unchanged, and there were no changes in calbindin D28k or synaptophysin 1 immunoreactivity after irradiation. We conclude that irradiation may result in a temporary loss of neuronal protein expression in mouse hippocampus. These changes do not necessarily indicate loss of neurons and indicate the need for caution regarding the use of phenotypic markers such as NeuN to estimate changes in neuronal numbers after irradiation.
ISSN:0022-3069
1554-6578
DOI:10.1097/NEN.0b013e3181d1afe4