Galectin-1 stimulates monocyte chemotaxis via the p44/42 MAP kinase pathway and a pertussis toxin-sensitive pathway

Galectin-1, the prototype of a family of β-galactoside-binding proteins, has been implicated in a wide variety of biological processes. Data presented herein show that galectin-1 stimulates monocyte migration in a dose-dependent manner but is not chemotactic for macrophages. Galectin-1-induced monoc...

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Bibliographic Details
Published in:Glycobiology (Oxford) 2009-12, Vol.19 (12), p.1402-1407
Main Authors: Malik, Reshad K.J, Ghurye, Rohit R, Lawrence-Watt, Diana J, Stewart, Helen J.S
Format: Article
Language:English
Subjects:
Cells, Cultured
chemotaxis
Chemotaxis, Leukocyte - drug effects
Drug Resistance - drug effects
Drug Resistance - physiology
Female
Galectin 1 - pharmacology
Galectin 1 - physiology
galectin-1
Humans
Inflammation - pathology
Lactose - pharmacology
Macrophages - drug effects
Macrophages - physiology
Male
Mitogen-Activated Protein Kinase 1 - metabolism
Mitogen-Activated Protein Kinase 1 - physiology
Mitogen-Activated Protein Kinase 3 - metabolism
Mitogen-Activated Protein Kinase 3 - physiology
monocytes
Monocytes - drug effects
Monocytes - physiology
Pertussis Toxin - pharmacology
signal transduction
Signal Transduction - drug effects
Signal Transduction - physiology
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Summary:Galectin-1, the prototype of a family of β-galactoside-binding proteins, has been implicated in a wide variety of biological processes. Data presented herein show that galectin-1 stimulates monocyte migration in a dose-dependent manner but is not chemotactic for macrophages. Galectin-1-induced monocyte chemotaxis is blocked by lactose and inhibited by an anti-galectin-1 antibody but not by nonspecific antibodies. Furthermore, galectin-1-mediated monocyte migration was significantly inhibited by MEK inhibitors in a rapid, time-dependent manner suggesting that MAP kinase pathways are involved in galectin-1. Migration was also almost completely blocked by pertussis toxin implying G-protein involvement in the galectin-1-induced chemotaxis. These results demonstrate a role for galectin-1 in monocyte chemotaxis which differs from galectin-3 in that macrophages are nonresponsive. Furthermore, our observations suggest that galectin-1 may be involved in chemoattraction at sites of inflammation in vivo and may contribute to disease processes such as atherosclerosis.
ISSN:0959-6658
1460-2423
DOI:10.1093/glycob/cwp077