Effect of donor cell types on developmental potential of cattle (Bos taurus) and swamp buffalo (Bubalus bubalis) cloned embryos

This study investigated the effect of donor cell types on the developmental potential and quality of cloned swamp buffalo embryos in comparison with cloned cattle embryos. Fetal fibroblasts (FFs), ear fibroblasts (EFs), granulosa cells (GCs) and cumulus cells (CCs) were used as the donor cells in bo...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2010, Vol.56(1), pp.49-54
Main Authors: Srirattana, K., Suranaree Univ. of Technology, Nakhon Ratchasima (Thailand), Lorthongpanich, C, Laowtammathron, C, Imsoonthornruksa, S, Ketudat-Cairns, M, Phermthai, T, Nagai, T, Parnpai, R
Format: Article
Language:English
Subjects:
ANIMAL BIOTECHNOLOGY
ANIMAL EMBRYOS
Animals
BIOTECHNOLOGIE ANIMALE
BIOTECNOLOGIA ANIMAL
Blastocyst - physiology
BOVIN
BUFALO DE AGUA
Buffaloes - embryology
BUFFLE D'EAU
CATTLE
Cattle - embryology
CELLS
CELLULE
CELULAS
CLONACION
CLONAGE
Clone Cells - physiology
CLONING
Cloning, Organism
Cumulus Cells - physiology
Differential staining
EMBRIONES ANIMALES
Embryo development
EMBRYON ANIMAL
Embryonic Development - physiology
Female
Fibroblasts - physiology
GANADO BOVINO
Granulosa Cells - physiology
http://www.fao.org/aos/agrovoc#c_1391
http://www.fao.org/aos/agrovoc#c_1418
http://www.fao.org/aos/agrovoc#c_15952
http://www.fao.org/aos/agrovoc#c_25388
http://www.fao.org/aos/agrovoc#c_27450
http://www.fao.org/aos/agrovoc#c_8313
Male
Morula - physiology
Nuclear transfer
Nuclear Transfer Techniques
Swamp buffalo
WATER BUFFALOES
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Summary:This study investigated the effect of donor cell types on the developmental potential and quality of cloned swamp buffalo embryos in comparison with cloned cattle embryos. Fetal fibroblasts (FFs), ear fibroblasts (EFs), granulosa cells (GCs) and cumulus cells (CCs) were used as the donor cells in both buffalo and cattle. The cloned cattle or buffalo embryos were produced by fusion of the individual donor cells with enucleated cattle or buffalo oocytes, respectively. The reconstructed (cloned) embryos and in vitro matured oocytes without enucleation were parthenogenetically activated (PA) and cultured for 7 days. Their developmental ability to the blastocyst stage was evaluated. The total number of trophectoderm (TE) and inner cell mass (ICM) cells and the ICM ratio in each blastocyst was determined by differential staining as an indicator of embryo quality. The fusion rate of CCs with enucleated oocytes was significantly lower than for those of other donor cell types both in cattle and buffalo. The rates of cleavage and development to the 8-cell, morula and blastocyst stages of cloned embryos derived from all donor cell types did not significantly differ within the same species. However, the cleavage rate of cloned cattle embryos derived from FFs was significantly higher than those of cattle PA and cloned buffalo embryos. The blastocyst rates of cloned cattle embryos, except for the ones derived from CCs, were significantly higher than those of cloned buffalo embryos. In buffalo, only cloned embryos derived from CCs showed a significantly higher blastocyst rate than that of PA embryos. In contrast, all the cloned cattle embryos showed significantly higher blastocyst rates than that of PA embryos. There was no difference in ICM ratio among any of the blastocysts derived from any of the donor cell types and PA embryos in both species. FFs, EFs, GCs and CCs had similar potentials to support development of cloned cattle and buffalo embryos to the blastocyst stage with the same quality.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.09-135A