Effects of Phytoestrogen Extracts Isolated from Flax on Estradiol Production and ER/PR Expression in MCF7 Breast Cancer Cells

In this study, we tested the effects of crude extracts from flax (Linum usitatissimum) on the production of estradiol and expression of estrogen receptor (ER) and progesterone receptor (PR) in human breast cancer MCF7 cells. Isoflavone and lignan extracts from flax plant Linum usitatissimum were obt...

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Published in:Anticancer research 2010-05, Vol.30 (5), p.1695-1699
Main Authors: RICHTER, D.-U, ABARZUA, S, JESCHKE, U, CHROBAK, M, SCHOLZ, C, KUHN, C, SCHULZE, S, KUPKA, M. S, FRIESE, K, BRIESE, V, PIECHULLA, B
Format: Article
Language:English
Subjects:
Biological and medical sciences
Breast Neoplasms - metabolism
Cell Line, Tumor
Cell Proliferation
Dose-Response Relationship, Drug
Estradiol - metabolism
Female
Flax - metabolism
Gene Expression Regulation, Neoplastic
Gynecology. Andrology. Obstetrics
Humans
Lignans - chemistry
Mammary gland diseases
Medical sciences
Phytoestrogens - chemistry
Phytoestrogens - metabolism
Plant Extracts - metabolism
Plant Roots - metabolism
Receptors, Estrogen - metabolism
Receptors, Progesterone - metabolism
Tumors
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Summary:In this study, we tested the effects of crude extracts from flax (Linum usitatissimum) on the production of estradiol and expression of estrogen receptor (ER) and progesterone receptor (PR) in human breast cancer MCF7 cells. Isoflavone and lignan extracts from flax plant Linum usitatissimum were obtained, using different extraction methods. Breast carcinoma cells (MCF7) were incubated with various concentrations of the isolated extracts. Untreated MCF7 cells were used as controls. Supernatants were removed at designated times and tested for estradiol with an ELISA method. Furthermore, the effect of phytoestrogen extracts on the production of ERa and ERbeta as well as on PR was examined. Production of estradiol is elevated in MCF7 cells in a concentration-dependent manner after stimulation with isoflavone and lignan extracts from Linum usitatissimum. Expression of ERalpha is up-regulated after stimulation with lower concentrations of lignan extracts from flax plants, unchanged at median concentrations and down-regulated at high concentrations. Expression of ERbeta is down-regulated in a concentration-dependent manner.
ISSN:0250-7005
1791-7530