Effect and Mechanism of Panaxoside Rg1 on Neovascularization in Myocardial Infarction Rats

Objective：To investigate the effects and mechanisms of panaxoside Rg1 on the new vessel formation in acute myocardial infarction（AMI） rats.Methods：The AMI model of male Sprague-Dawley（SD） rats was established,and rats were randomly divided into the AMI model group,the treatment group of panaxoside R...

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Published in:Chinese journal of integrative medicine 2010-04, Vol.16 (2), p.162-166
Main Author: 王孝东 谷天祥 师恩祎 卢春茂 王春
Format: Article
Language:English
Subjects:
Animals
Cell Count
Collateral Circulation - drug effects
Drug Evaluation, Preclinical
Experimental Research
Ginsenosides - administration & dosage
Ginsenosides - pharmacology
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Intracellular Signaling Peptides and Proteins - metabolism
Intracellular Signaling Peptides and Proteins - physiology
Male
Medicine
Medicine & Public Health
Microvessels - pathology
Myocardial Infarction - drug therapy
Myocardial Infarction - metabolism
Myocardial Infarction - pathology
Neovascularization, Physiologic - drug effects
Placebos
Platelet Endothelial Cell Adhesion Molecule-1 - metabolism
Protein-Serine-Threonine Kinases - metabolism
Protein-Serine-Threonine Kinases - physiology
Random Allocation
Rats
Rats, Sprague-Dawley
Signal Transduction - drug effects
Sirolimus - administration & dosage
Sirolimus - pharmacology
TOR Serine-Threonine Kinases
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Summary:Objective：To investigate the effects and mechanisms of panaxoside Rg1 on the new vessel formation in acute myocardial infarction（AMI） rats.Methods：The AMI model of male Sprague-Dawley（SD） rats was established,and rats were randomly divided into the AMI model group,the treatment group of panaxoside Rg1,the placebo group and the treatment group of panaxoside Rg1 plus rapamycin.Cardiac creatases were determined with 1 mL blood drawn from vena caudalis of the rats 48 h after the model was successfully made. After 4 weeks,Evans blue was injected into the aorta roots of the rats,and then,red tetrazoline was dyed again and the myocardial infarction area was evaluated.The microvessel density（MVD） of infarction area was determined by the immunohistochemistry of CD31;enzyme-linked immunosorbent assay（ELISA） was used to detect the protein content of CD31 and hypoxia inducible factor-1α（HIF-1α） of the infarction area. Results：The MVD in the infarction area and the contents of CD31 and HIF-1αin the Rg1 treatment group were higher than those in the AMI model group significantly（P〈0.05）.The cardiac creatase and infarction area were lower in the Rg1 treatment group than those in the AMI model group significantly（P〈0.05）.The above effects,however,disappeared when rapamycin,the antagonist of mammalian target of rapamycin（mTOR）,was administered simultaneously.Conclusions：Panaxoside Rg1 could increase the expression of HIF-1αand CD31 of myocardium and stimulate the angiogenesis.The above mentioned role of panaxoside Rg1 might be related to the excitation of mTOR receptor.
ISSN:1672-0415
1993-0402
DOI:10.1007/s11655-010-0162-4