Lipid characterization of an enriched plasma membrane fraction of Dunaliella salina grown in media of varying salinity

We have developed a rapid procedure for isolating a fraction enriched in plasma membrane from Dunaliella salina using an aqueous two-phase system (dextran/polyethylene glycol, 6.7%/6.7%). An enriched plasma membrane fraction, free of chloroplast and mitochondrial contamination, could be obtained in...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) 1989-03, Vol.89 (3), p.970-976
Main Authors: Peeler, T.C, Stephenson, M.B, Einspahr, K.J, Thompson, G.A. Jr
Format: Article
Language:English
Subjects:
ALGAE
ALGUE
ANALISIS
ANALYSE
biochemical phenomena
Biological and medical sciences
Cell biochemistry
Cell growth
Cell membranes
Cell physiology
Chloroplasts
COMPOSICION
COMPOSITION
CONTENIDO DE LIPIDOS
Dunaliella
Dunaliella salina
ESTRUCTURA CELULAR
Fatty acids
Fundamental and applied biological sciences. Psychology
INDICE DE CRECIMIENTO
inositol phospholipids
Lipids
Marine
MEDIO DE CULTIVO
methodology
MILIEU DE CULTURE
Peroxides
phosphatidylcholine
phosphatidylethanolamine
Phospholipids
plant physiology
Plant physiology and development
plasma membranes
Plasma physics
PROTEINAS
PROTEINE
SALINIDAD
SALINITE
salinity
Sterols
STRUCTURE CELLULAIRE
TAUX DE CROISSANCE
TENEUR EN LIPIDES
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed a rapid procedure for isolating a fraction enriched in plasma membrane from Dunaliella salina using an aqueous two-phase system (dextran/polyethylene glycol, 6.7%/6.7%). An enriched plasma membrane fraction, free of chloroplast and mitochondrial contamination, could be obtained in 2.5 hours. Plasma membrane proteins, which accounted for approximately 1% of the total membrane protein, contained a number of unique proteins compared with the other cell fractions, as shown by gel electrophoresis. The lipids of the plasma membrane fraction from 1.7 molar NaCl-grown cells were extracted and characterized. Phosphatidylethanolamine and phosphatidycholine were the two most prevalent phospholipids, at 20.6% and 6.0% of the total lipid, respectively. In addition, inositol phospholipids were a significant component of the D. salina plasma membrane fraction. Phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate accounted for 5.2% and 1.5% of the plasma membrane phospholipid, respectively. Diacylglyceryltrimethylhomoserine accounted for 7.9% of the plasma membrane total lipid. Free sterols were the major component of the plasma membrane fraction, at 55% of the total lipid, and consisted of ergosterol and 7-dehydroporiferasterol. Sterol peroxides were not present in the plasma membrane fraction. The lipid composition of enriched plasma membrane fractions from cells grown at 0.85 molar NaCl and 3.4 molar NaCl were compared with those grown a t 1.7 molar NaCl. The concentration of diacylglyceryltrimethylhomoserine and the degree of plasma membrane fatty acid saturation increased in 3.4 molar plasma membranes. The relative concentration of sterols in the plasma membrane fraction was similar in all three NaCl concentrations tested
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.89.3.970