Properties of a Mutant from Synechocystis PCC6803 Resistant to Acetazolamide, an Inhibitor of Carbonic Anhydrase

A spontaneous mutant of the cyanobacterium Synechocystis PCC6803 was isolated for its resistance to acetazolamide, an inhibitor of carbonic anhydrase. The mutant showed a deficiency in oxygen exchange between CO2 and H2O, a lower level of stable internal CO2 pool and a decreased capacity to adapt it...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1990-08, Vol.93 (4), p.1312-1315
Main Authors: Bédu, Sylvie, Peltier, Gilles, Frédéric Sarrey, Françoise Joset
Format: Article
Language:English
Subjects:
Active transport
Biological and medical sciences
Carbon dioxide
Cell growth
Cell structures and functions
Chloroplast, photosynthetic membrane and photosynthesis
Cyanobacteria
Enzymes
Freshwater
Fundamental and applied biological sciences. Psychology
Mass spectrometers
Mass spectroscopy
Molecular and cellular biology
Photosynthesis
Plant cells
Plants
Synechocystis
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Summary:A spontaneous mutant of the cyanobacterium Synechocystis PCC6803 was isolated for its resistance to acetazolamide, an inhibitor of carbonic anhydrase. The mutant showed a deficiency in oxygen exchange between CO2 and H2O, a lower level of stable internal CO2 pool and a decreased capacity to adapt its photo-synthetic affinity under limited inorganic carbon regime. The initial rate of uptake of inorganic carbon was identical to that of wild-type cells. It is demonstrated that the mutation affects the carbonic anhydrase activity. This could result from either of two impairments: a deficiency in the enzyme activity detectable by mass spectrometric determinations, or a modification of the cellular compartment in which the enzyme is located, preventing its activity.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.93.4.1312