Helminthosporium maydis race T toxin induces leakage of NAD+ from T cytoplasm corn mitochondria

The mechanism by which Helminthosporium maydis race T toxin inhibits respiration dependent on NAD+-linked substrates in T cytoplasm corn mitochondria was investigated. The toxin did not cause leakage of the soluble matrix enzyme malate dehydrogenase from the mitochondria or inhibit malate dehydrogen...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1979-06, Vol.63 (6), p.1149-1153
Main Authors: Matthews, David E., Gregory, Peter, Vernon E. Gracen
Format: Article
Language:English
Subjects:
Corn
Cytoplasm
Dehydrogenases
Helminthosporium
Isocitrates
Mitochondria
Oxidative phosphorylation
Respiration
Swelling
Toxins
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Summary:The mechanism by which Helminthosporium maydis race T toxin inhibits respiration dependent on NAD+-linked substrates in T cytoplasm corn mitochondria was investigated. The toxin did not cause leakage of the soluble matrix enzyme malate dehydrogenase from the mitochondria or inhibit malate dehydrogenase or isocitrate dehydrogenase directly. The toxin did increase the permeability of the inner membranes of T cytoplasm, but not N cytoplasm, mitochondria to NAD+. Added NAD+ partially or fully restored toxin-inhibited electron transport in T cytoplasm mitochondria. Thiamin pyrophosphate had a similar effect when malate was the substrate. It was concluded that the inhibition of respiration of NAD+-linked substrates by the toxin is due to depletion of the intramitochondrial pool of NAD+ and other coenzymes.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.63.6.1149