Investigation of stratum corneum lipid model membranes with free fatty acid composition by neutron diffraction

The outermost epidermal layer, the stratum corneum (SC), is the main skin barrier. Studies of SC model systems enable characterization of the influence of individual lipids on the organization of the SC lipid matrix, which is the main pathway of water through the skin. This work presents a neutron d...

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Bibliographic Details
Published in:European biophysics journal 2010-07, Vol.39 (8), p.1167-1176
Main Authors: Ryabova, Natalia Yu, Kiselev, M. A, Dante, S, Hauß, T, Balagurov, A. M
Format: Article
Language:English
Subjects:
Algorithms
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Cell Biology
Cell Membrane - chemistry
Cellular biology
Ceramides - chemistry
Cholesterol - chemistry
Cholesterol Esters - chemistry
Diffraction
Epidermis - chemistry
Fatty acids
Fatty Acids - chemistry
Fatty Acids, Nonesterified - chemistry
Humans
Hydration
Hydrophobic and Hydrophilic Interactions
Kinetics
Life Sciences
Lipids
Membrane Biology
Membranes
Membranes, Artificial
Model lipid membrane
Models, Chemical
Nanotechnology
Neurobiology
Neutron Diffraction
Original Paper
Palmitic Acid - chemistry
Skin
Stratum corneum
Temperature
Water - chemistry
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Summary:The outermost epidermal layer, the stratum corneum (SC), is the main skin barrier. Studies of SC model systems enable characterization of the influence of individual lipids on the organization of the SC lipid matrix, which is the main pathway of water through the skin. This work presents a neutron diffraction study of the SC model membranes based on short-chain ceramide 6 with nearly realistic composition of free fatty acids (FFA) at physiological temperature of the SC. The influence of FFA and the effect of cholesterol-cholesterol sulfate substitution on the structure and hydration of the SC model membranes are described. The structure of the SC membrane with FFA is close to the structure of the earlier studied SC membrane based on short-chain palmitic acid (PA) and does not vary significantly under changes of the ratio of the main membrane components. FFA accelerates membrane swelling at the same low level of hydration of both PA- and FFA-containing membranes. The substitution of cholesterol sulfate by cholesterol in the membrane composition decreases membrane swelling and leads to phase separation in the model system.
ISSN:0175-7571
1432-1017
DOI:10.1007/s00249-009-0569-z