Determination of mutacin activity and detection of mutA genes in Streptococcus mutans genotypes from caries-free and caries-active children

Relationships between genetic diversity, mutacin production and sensitivity to mutacins in Streptococcus mutans were evaluated in 19 clinical isolates from caries‐free and caries‐active children. Mutacin production was tested against 30 indicator strains; results showed significant variations in the...

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Bibliographic Details
Published in:Oral microbiology and immunology 2003-06, Vol.18 (3), p.144-149
Main Authors: Longo, P. L., Mattos-Graner, R. O., Mayer, M. P. A.
Format: Article
Language:English
Subjects:
Bacteriocins - genetics
Biological and medical sciences
Child, Preschool
Dental Caries - microbiology
Dentistry
DNA, Bacterial - analysis
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
genotyping
Humans
Infant
Microbiology
mutacin
Random Amplified Polymorphic DNA Technique
Statistics, Nonparametric
Streptococcus mutans
Streptococcus mutans - genetics
Streptococcus mutans - pathogenicity
Virulence
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Summary:Relationships between genetic diversity, mutacin production and sensitivity to mutacins in Streptococcus mutans were evaluated in 19 clinical isolates from caries‐free and caries‐active children. Mutacin production was tested against 30 indicator strains; results showed significant variations in the inhibitory spectra of the clinical isolates. There was no association between the inhibitory spectrum of the infecting strain and the caries experience or the level of mutans streptococci infection of the host. Homology to the mutA gene coding for mutacin II was detected in one clinical isolate; none of the clinical isolates showed homology to the mutA genes coding for mutacins I or III. Genotyping by random amplified polymorphic DNA (RAPD) reactions grouped the isolates into three clusters, but no correlation was found between any of the clusters and mutacin activity, caries experience or level of mutans streptococci in the host.
ISSN:0902-0055
1399-302X
DOI:10.1034/j.1399-302X.2003.00042.x