Myoglobin Redox Form Stabilization by Compartmentalized Lactate and Malate Dehydrogenases

The purpose of this study was to assess the ability of mitochondrial and cytoplasmic malate dehydrogenase present in postrigor bovine skeletal muscle to use malate as a substrate for reduced nicotinamide adenine dinucleotide (NADH) regeneration and metmyoglobin (MMb) reduction via the malate−NAD+−MM...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2010-06, Vol.58 (11), p.7021-7029
Main Authors: Mohan, Anand, Hunt, Melvin C, Muthukrishnan, S, Barstow, Thomas J, Houser, Terry A
Format: Article
Language:English
Subjects:
Animals
beef
Biological and medical sciences
Cattle
color
cytoplasm
Cytoplasm - chemistry
Cytoplasm - enzymology
Cytoplasm - metabolism
Enzyme Stability
Food Chemistry/Biochemistry
Food industries
Fundamental and applied biological sciences. Psychology
Horses
lactic acid
Lactic Acid - metabolism
malate dehydrogenase
Malate Dehydrogenase - chemistry
Malate Dehydrogenase - metabolism
malic acid
Meat and meat product industries
metmyoblobin
mitochondria
Mitochondria - chemistry
Mitochondria - enzymology
Mitochondria - metabolism
muscle tissues
Muscle, Skeletal - chemistry
Muscle, Skeletal - enzymology
Muscle, Skeletal - metabolism
myoglobin
Myoglobin - chemistry
Myoglobin - metabolism
NAD (coenzyme)
NAD - metabolism
Oxidation-Reduction
redox potential
reduction
skeletal muscle
Substrate Specificity
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Summary:The purpose of this study was to assess the ability of mitochondrial and cytoplasmic malate dehydrogenase present in postrigor bovine skeletal muscle to use malate as a substrate for reduced nicotinamide adenine dinucleotide (NADH) regeneration and metmyoglobin (MMb) reduction via the malate−NAD+−MMb system. Furthermore, addition of lactate to beef mitochondrial and cytoplasmic isolates was evaluated to determine whether interactions between malate and lactate increased MMb reduction. Addition of malate to isolated beef mitochondrial and cytoplasmic isolates at pH 7.2 increased (p < 0.05) MMb reduction. MMb reduction resulting from addition of malate and lactate was equal to or greater than MMb reduction resulting from malate alone. This suggests that a combination of mitochondrial (malate) and cytoplasmic (lactate) factors can be used to regenerate the post-mortem pool of NADH, resulting in metmyoglobin reduction and meat color stabilization.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf100714g