In vivo labelling of resting monocytes in the reticuloendothelial system with fluorescent iron oxide nanoparticles prior to injury reveals that they are mobilized to infarcted myocardium

Aims To evaluate the feasibility of loading resting monocytes/macrophages by intravenous (i.v.) injection of fluorescent iron oxide nanoparticles prior to injury and tracking of these cells in the very same animal to myocardial infarction (MI) by magnetic resonance imaging (MRI) and optical imaging....

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Published in:European heart journal 2010-06, Vol.31 (11), p.1410-1420
Main Authors: Montet-Abou, Karin, Daire, Jean-Luc, Hyacinthe, Jean-Noël, Jorge-Costa, Manuel, Grosdemange, Kerstin, Mach, François, Petri-Fink, Alke, Hofmann, Heinrich, Morel, Denis R., Vallée, Jean-Paul, Montet, Xavier
Format: Article
Language:English
Subjects:
Animals
Antigens, CD
Antigens, Differentiation, Myelomonocytic
Cell Movement - physiology
Ferric Compounds
Fluorescence
Inflammation
Iron oxide nanoparticles
Macrophages - metabolism
Macrophages - physiology
Magnetic Resonance Imaging
Metal Nanoparticles
Monocytes - metabolism
Monocytes - physiology
Mononuclear Phagocyte System
Myocardial infarction
Myocardial Infarction - diagnosis
Myocardial Reperfusion Injury - diagnosis
Rats
Rats, Wistar
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Summary:Aims To evaluate the feasibility of loading resting monocytes/macrophages by intravenous (i.v.) injection of fluorescent iron oxide nanoparticles prior to injury and tracking of these cells in the very same animal to myocardial infarction (MI) by magnetic resonance imaging (MRI) and optical imaging. Methods and results Rats were injected with fluorescent iron oxide nanoparticles (10 mg/kg) (n = 15) prior to injury. After disappearance of the nanoparticles from the blood, MI was induced. Monocytes/macrophages were then tracked in the very same animal by MRI and optical imaging. Control groups were (i) non-injected animals (n = 15), (ii) injected animals associated with a sham operation (n = 8), and (iii) animals treated with an anti-inflammatory agent (n = 6). The presence of iron-loaded cells can be detected by MRI in vivo in the infarcted myocardium. Here, we showed that the detection of inflammatory cells in vivo correlated well with ex vivo imaging (MRI and reflectance fluorescence) and histology. We also showed that the method is robust enough to depict changes in the inflammatory response. Conclusion This study demonstrates that resting monocytes/macrophages can be loaded in vivo by a simple i.v. injection of fluorescent superparamagnetic iron oxide nanoparticles prior to injury and then tracked, in the same animal, in a model of ischaemia–reperfusion leading to myocardial infarct. Although previous studies of macrophages infiltration following MI have labelled the macrophages after injury, this study, for the first time, has pre-load the resting monocytes with fluorescent iron oxide nanoparticles.
ISSN:0195-668X
1522-9645
DOI:10.1093/eurheartj/ehp547