Development and validation of a liquid chromatographic–tandem mass spectrometric method for the determination of galantamine in human heparinised plasma

Galantamine is an acetylcholinesterase inhibitor, recently approved for the treatment of mild-to-moderate Alzheimer’s disease. To allow a higher throughput of samples, a new bioanalytical method for the determination of galantamine in human plasma was developed and validated. A stable isotope labell...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-06, Vol.789 (2), p.337-346
Main Authors: Verhaeghe, T, Diels, L, de Vries, R, De Meulder, M, de Jong, J
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Galantamine
Galantamine - blood
General pharmacology
Heparin
Humans
Mass Spectrometry - methods
Medical sciences
Pharmacology. Drug treatments
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Galantamine is an acetylcholinesterase inhibitor, recently approved for the treatment of mild-to-moderate Alzheimer’s disease. To allow a higher throughput of samples, a new bioanalytical method for the determination of galantamine in human plasma was developed and validated. A stable isotope labelled internal standard was used. Sample preparation consisted of a simple one-step liquid–liquid extraction with toluene. The extracts were analysed with positive ion TurboIonspray tandem mass spectrometry (LC–MS–MS). The method was validated in the 1–500-ng/ml range. The accuracy, precision, selectivity, lower limit of quantification, upper limit of quantification, linearity and extraction recovery were evaluated, as well as the stability of the compound in plasma, blood, methanol and 2% BSA solutions under different conditions. The method proved very rugged during the analysis of large numbers of samples from clinical trials.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(03)00129-6