An infection control protocol: effectiveness of immersion solutions to reduce the microbial growth on dental prostheses

summary  This investigation evaluated the effectiveness of an infection control protocol for cleansing and disinfecting removable dental prostheses. Sixty‐four dentures were rubbed with sterile cotton swab immediately after they had been taken from patients' mouths. Samples were individually pl...

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Bibliographic Details
Published in:Journal of oral rehabilitation 2003-05, Vol.30 (5), p.532-536
Main Authors: Pavarina, A. C., Pizzolitto, A. C., Machado, A. L., Vergani, C. E., Giampaolo, E. T.
Format: Article
Language:English
Subjects:
Anti-Infective Agents, Local
Chlorhexidine - analogs & derivatives
Chlorhexidine - analysis
cross-contamination
Dental Prosthesis
Dentistry
denture base acrylic resin
Denture Cleansers
disinfection
Disinfection - methods
Equipment Contamination - prevention & control
Female
Humans
Male
removable prosthodontics
Sodium Hypochlorite - analysis
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Summary:summary  This investigation evaluated the effectiveness of an infection control protocol for cleansing and disinfecting removable dental prostheses. Sixty‐four dentures were rubbed with sterile cotton swab immediately after they had been taken from patients' mouths. Samples were individually placed in the culture medium and immediately incubated at 37 ± 2 °C. The dentures were scrubbed for 1 min with 4% chlorhexidine, rinsed for 1 min in sterile water and placed for 10 min in one of the following immersion solutions: 4% chlorhexidine gluconate, 1% sodium hypochlorite, Biocide (iodophors) and Amosan (alkaline peroxide). After the disinfection procedures, the dentures were immersed in sterile water for 3 min, reswabbed and the samples were incubated. All samples obtained in the initial culture were contaminated with micro‐organisms. All the lower dentures immersed in Biocide showed positive growth, and the upper dentures were positive for growth in six of eight dentures. The 4% chlorhexidine gluconate, 1% sodium hypochlorite and Amosan solutions have been proved effective to reduce the growth of the micro‐organisms in the 10 min immersion period. The protocol evaluated in this study seems to be a viable method to prevent cross‐contamination between dental personnel and patients.
ISSN:0305-182X
1365-2842
DOI:10.1046/j.1365-2842.2003.01093.x