Early IL-2 Production by Mouse Dendritic Cells Is the Result of Microbial-Induced Priming

Dendritic cells (DCs) are professional APCs able to initiate innate and adaptive immune responses against invading pathogens. Different properties such as the efficient Ag processing machinery, the high levels of expression of costimulatory molecules and peptide-MHC complexes, and the production of...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2003-05, Vol.170 (10), p.5075-5081
Main Authors: Granucci, Francesca, Feau, Sonia, Angeli, Veronique, Trottein, Francois, Ricciardi-Castagnoli, Paola
Format: Article
Language:English
Subjects:
Animals
Cell Communication - immunology
Cell Line
Cells, Cultured
Dendritic Cells - immunology
Dendritic Cells - metabolism
Dendritic Cells - microbiology
Escherichia coli - immunology
Immunization - methods
Injections, Intraperitoneal
Interleukin-2 - biosynthesis
Interleukin-2 - metabolism
Lipopolysaccharides - administration & dosage
Lipopolysaccharides - immunology
Lymphoid Tissue - immunology
Lymphoid Tissue - metabolism
Melanoma, Experimental
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Peptidoglycan - immunology
Phagocytosis - immunology
T-Lymphocytes - immunology
Teichoic Acids - immunology
Tumor Cells, Cultured
Zymosan - immunology
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Summary:Dendritic cells (DCs) are professional APCs able to initiate innate and adaptive immune responses against invading pathogens. Different properties such as the efficient Ag processing machinery, the high levels of expression of costimulatory molecules and peptide-MHC complexes, and the production of cytokines contribute in making DCs potent stimulators of naive T cell responses. Recently we have observed that DCs are able to produce IL-2 following bacterial stimulation, and we have demonstrated that this particular cytokine is a key molecule conferring to early bacterial activated DCs unique T cell priming capacity. In the present study we show that many different microbial stimuli, but not inflammatory cytokines, are able to stimulate DCs to produce IL-2, indicating that DCs can distinguish a cytokine-mediated inflammatory process from the actual presence of an infection. The capacity to produce IL-2 following a microbial stimuli encounter is a feature shared by diverse DC subtypes in vivo, such as CD8 alpha(+) and CD8 alpha(-) splenic DCs and epidermal Langerhans cells. When early activated DCs interact with T cells, IL-2 produced by DCs is enriched at the site of cell-cell contact, confirming the importance of DCs-derived IL-2 in T cell activation.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.170.10.5075