Cytochrome c location in phosphatidylcholine/cardiolipin model membranes: resonance energy transfer study

Resonance energy transfer between lipid-bound fluorescent probe 3-methoxybenzanthrone as a donor and heme group of cytochrome c as an acceptor has been examined to ascertain the protein disposition relative to the surface of model membranes composed of phosphatidylcholine and cardiolipin (10, 50 and...

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Bibliographic Details
Published in:Biophysical chemistry 2003-03, Vol.103 (3), p.239-249
Main Authors: Gorbenko, Galina P, Domanov, Yegor A
Format: Article
Language:English
Subjects:
Animals
Benz(a)Anthracenes - chemistry
Cardiolipins - chemistry
Cardiolipins - metabolism
Cattle
Cytochrome c
Cytochrome c Group - chemistry
Cytochrome c Group - metabolism
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemistry
Heme - chemistry
Lipid Bilayers - chemistry
Membranes, Artificial
Models, Biological
Models, Chemical
Phosphatidylcholines - chemistry
Phosphatidylcholines - metabolism
Protein bilayer location
Protein–lipid complexes
Quantum Theory
Resonance energy transfer
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Summary:Resonance energy transfer between lipid-bound fluorescent probe 3-methoxybenzanthrone as a donor and heme group of cytochrome c as an acceptor has been examined to ascertain the protein disposition relative to the surface of model membranes composed of phosphatidylcholine and cardiolipin (10, 50 and 80 mol%). The model of energy transfer in membrane systems has been extended to the case of donors distributed between the two-bilayer leaflets and acceptors located at the outer monolayer taking into account the donor and acceptor orientational behavior. Assuming specific protein orientation relative to the membrane surface and varying lateral distance of the donor–acceptor closest approach in the range from 0 to 3.5 nm the limits for possible heme distances from the bilayer midplane have been found to be 0.8–3 nm (10 mol% CL), 0–2.6 nm (50 mol% CL), and 1.4–3.3 nm (80 mol% CL).
ISSN:0301-4622
1873-4200
DOI:10.1016/S0301-4622(02)00319-8