The collagenase activity of Porphyromonas gingivalis is due to Arg-gingipain

Degradation of type I collagen by Porphyromonas gingivalis was monitored by fluorogenic, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), and growth assays. All three assays showed that inactivation of both the rgpA and rgpB genes was necessary to completely eliminate the capaci...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 2003-04, Vol.221 (2), p.181-185
Main Authors: Houle, Marie-Andrée, Grenier, Daniel, Plamondon, Pascale, Nakayama, Koji
Format: Article
Language:English
Subjects:
Adhesins, Bacterial
Biological and medical sciences
Collagen - metabolism
Collagenase
Collagenases - metabolism
Cysteine Endopeptidases - metabolism
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Gingipain
Hemagglutinins - metabolism
Microbiology
Periodontitis
Periodontitis - microbiology
Porphyromonas gingivalis
Porphyromonas gingivalis - drug effects
Porphyromonas gingivalis - enzymology
Porphyromonas gingivalis - growth & development
Protease Inhibitors - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Degradation of type I collagen by Porphyromonas gingivalis was monitored by fluorogenic, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), and growth assays. All three assays showed that inactivation of both the rgpA and rgpB genes was necessary to completely eliminate the capacity of P. gingivalis to cleave type I collagen. Leupeptin, an Arg-gingipain-specific protease inhibitor, almost completely inhibited collagen degradation by P. gingivalis cells whereas cathepsin B inhibitor II, a Lys-gingipain inhibitor, did not. A purified preparation of Arg-gingipains A and B hydrolyzed gelatin but did not cleave type I collagen, suggesting that the enzymes must be attached to the cell surface to exert collagenase activity. A number of substances used as adjuncts in periodontal therapy were also tested for their capacity to inhibit collagenase activity of P. gingivalis. Tetracycline, doxycycline, and chlorhexidine strongly inhibited collagenase activity.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00178-2